Loading…

Drug resistance evaluation in novel 3D in vitro model

Ovarian cancer rates the highest mortality among all gynecological malignancies. The main reason for high mortality is the development of drug resistance. It can be related to changes in the expression of many drug resistance genes as well as expression of extracellular matrix proteins and cell dens...

Full description

Saved in:
Bibliographic Details
Published in:Biomedicine & pharmacotherapy 2021-06, Vol.138, p.111536-111536, Article 111536
Main Authors: Nowacka, Marta, Sterzynska, Karolina, Andrzejewska, Malgorzata, Nowicki, Michal, Januchowski, Radoslaw
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c385t-9d85b312e17689520538ccb5e32cce61c1fa3735d348f98577ab318cc83636593
cites cdi_FETCH-LOGICAL-c385t-9d85b312e17689520538ccb5e32cce61c1fa3735d348f98577ab318cc83636593
container_end_page 111536
container_issue
container_start_page 111536
container_title Biomedicine & pharmacotherapy
container_volume 138
creator Nowacka, Marta
Sterzynska, Karolina
Andrzejewska, Malgorzata
Nowicki, Michal
Januchowski, Radoslaw
description Ovarian cancer rates the highest mortality among all gynecological malignancies. The main reason for high mortality is the development of drug resistance. It can be related to changes in the expression of many drug resistance genes as well as expression of extracellular matrix proteins and cell density in the tumor. We developed a simple two-dimensional and three-dimensional model of drug sensitive A2780 and resistant to cisplatin and paclitaxel variants of ovarian cancer cell line. Using MTT assay, we compared drug resistance in two-dimensional and three-dimensional cell culture conditions. Real-time polymerase chain reaction analysis was used to compare the expression of drug resistance genes. The expression of proteins in spheroids was determined by immunohistochemistry. We observed a moderate increase in cisplatin resistance and a significant increase in paclitaxel resistance between two-dimensional and three-dimensional cell culture conditions. Our findings show that changes in the expression of drug resistance genes may play a crucial role in the drug resistance of cancer cells in traditional cell culture. On the other hand, the drug resistance in spheroids may result from different mechanisms such as cell density in the spheroid, extracellular matrix proteins expression and drug capacity to diffuse into the spheroid. [Display omitted] •Resistance to cisplatin and paclitaxel in two-dimensional and three-dimensional cell culture conditions was examined.•In two-dimensional cell culture, changes in the genes expression might be the pivotal mechanism leading to drug resistance.•In three-dimensional cell culture, tissue-specific mechanisms might be crucial in drug resistance development.
doi_str_mv 10.1016/j.biopha.2021.111536
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2555642129</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0753332221003218</els_id><sourcerecordid>2555642129</sourcerecordid><originalsourceid>FETCH-LOGICAL-c385t-9d85b312e17689520538ccb5e32cce61c1fa3735d348f98577ab318cc83636593</originalsourceid><addsrcrecordid>eNp9kMtOwzAQRS0EEuXxByyyZJNgezqOs0FCLS-pEhtYW64zAVdpXOwkEn9PqrBmNXdx7pXmMHYjeCG4UHe7YuvD4csWkktRCCEQ1AlbiAp5rjgvT9mClwg5gJTn7CKlHeccFegFw3UcPrNIyafedo4yGm072N6HLvNd1oWR2gzWxzz6PoZsH2pqr9hZY9tE13_3kn08Pb6vXvLN2_Pr6mGTO9DY51WtcQtCkiiVrlByBO3cFgmkc6SEE42FErCGpW4qjWVpJ3xCNChQWMElu513DzF8D5R6s_fJUdvajsKQjEREtZRCHtHljLoYUorUmEP0ext_jODmaMnszGzJHC2Z2dJUu59rNL0xeoomOU-TiNpHcr2pg_9_4BfScW_N</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2555642129</pqid></control><display><type>article</type><title>Drug resistance evaluation in novel 3D in vitro model</title><source>Elsevier:Jisc Collections:Elsevier Read and Publish Agreement 2022-2024:Freedom Collection (Reading list)</source><creator>Nowacka, Marta ; Sterzynska, Karolina ; Andrzejewska, Malgorzata ; Nowicki, Michal ; Januchowski, Radoslaw</creator><creatorcontrib>Nowacka, Marta ; Sterzynska, Karolina ; Andrzejewska, Malgorzata ; Nowicki, Michal ; Januchowski, Radoslaw</creatorcontrib><description>Ovarian cancer rates the highest mortality among all gynecological malignancies. The main reason for high mortality is the development of drug resistance. It can be related to changes in the expression of many drug resistance genes as well as expression of extracellular matrix proteins and cell density in the tumor. We developed a simple two-dimensional and three-dimensional model of drug sensitive A2780 and resistant to cisplatin and paclitaxel variants of ovarian cancer cell line. Using MTT assay, we compared drug resistance in two-dimensional and three-dimensional cell culture conditions. Real-time polymerase chain reaction analysis was used to compare the expression of drug resistance genes. The expression of proteins in spheroids was determined by immunohistochemistry. We observed a moderate increase in cisplatin resistance and a significant increase in paclitaxel resistance between two-dimensional and three-dimensional cell culture conditions. Our findings show that changes in the expression of drug resistance genes may play a crucial role in the drug resistance of cancer cells in traditional cell culture. On the other hand, the drug resistance in spheroids may result from different mechanisms such as cell density in the spheroid, extracellular matrix proteins expression and drug capacity to diffuse into the spheroid. [Display omitted] •Resistance to cisplatin and paclitaxel in two-dimensional and three-dimensional cell culture conditions was examined.•In two-dimensional cell culture, changes in the genes expression might be the pivotal mechanism leading to drug resistance.•In three-dimensional cell culture, tissue-specific mechanisms might be crucial in drug resistance development.</description><identifier>ISSN: 0753-3322</identifier><identifier>EISSN: 1950-6007</identifier><identifier>DOI: 10.1016/j.biopha.2021.111536</identifier><language>eng</language><publisher>Elsevier Masson SAS</publisher><subject>Drug resistance ; Ovarian cancer ; Spheroids</subject><ispartof>Biomedicine &amp; pharmacotherapy, 2021-06, Vol.138, p.111536-111536, Article 111536</ispartof><rights>2021 The Authors</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-9d85b312e17689520538ccb5e32cce61c1fa3735d348f98577ab318cc83636593</citedby><cites>FETCH-LOGICAL-c385t-9d85b312e17689520538ccb5e32cce61c1fa3735d348f98577ab318cc83636593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Nowacka, Marta</creatorcontrib><creatorcontrib>Sterzynska, Karolina</creatorcontrib><creatorcontrib>Andrzejewska, Malgorzata</creatorcontrib><creatorcontrib>Nowicki, Michal</creatorcontrib><creatorcontrib>Januchowski, Radoslaw</creatorcontrib><title>Drug resistance evaluation in novel 3D in vitro model</title><title>Biomedicine &amp; pharmacotherapy</title><description>Ovarian cancer rates the highest mortality among all gynecological malignancies. The main reason for high mortality is the development of drug resistance. It can be related to changes in the expression of many drug resistance genes as well as expression of extracellular matrix proteins and cell density in the tumor. We developed a simple two-dimensional and three-dimensional model of drug sensitive A2780 and resistant to cisplatin and paclitaxel variants of ovarian cancer cell line. Using MTT assay, we compared drug resistance in two-dimensional and three-dimensional cell culture conditions. Real-time polymerase chain reaction analysis was used to compare the expression of drug resistance genes. The expression of proteins in spheroids was determined by immunohistochemistry. We observed a moderate increase in cisplatin resistance and a significant increase in paclitaxel resistance between two-dimensional and three-dimensional cell culture conditions. Our findings show that changes in the expression of drug resistance genes may play a crucial role in the drug resistance of cancer cells in traditional cell culture. On the other hand, the drug resistance in spheroids may result from different mechanisms such as cell density in the spheroid, extracellular matrix proteins expression and drug capacity to diffuse into the spheroid. [Display omitted] •Resistance to cisplatin and paclitaxel in two-dimensional and three-dimensional cell culture conditions was examined.•In two-dimensional cell culture, changes in the genes expression might be the pivotal mechanism leading to drug resistance.•In three-dimensional cell culture, tissue-specific mechanisms might be crucial in drug resistance development.</description><subject>Drug resistance</subject><subject>Ovarian cancer</subject><subject>Spheroids</subject><issn>0753-3322</issn><issn>1950-6007</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kMtOwzAQRS0EEuXxByyyZJNgezqOs0FCLS-pEhtYW64zAVdpXOwkEn9PqrBmNXdx7pXmMHYjeCG4UHe7YuvD4csWkktRCCEQ1AlbiAp5rjgvT9mClwg5gJTn7CKlHeccFegFw3UcPrNIyafedo4yGm072N6HLvNd1oWR2gzWxzz6PoZsH2pqr9hZY9tE13_3kn08Pb6vXvLN2_Pr6mGTO9DY51WtcQtCkiiVrlByBO3cFgmkc6SEE42FErCGpW4qjWVpJ3xCNChQWMElu513DzF8D5R6s_fJUdvajsKQjEREtZRCHtHljLoYUorUmEP0ext_jODmaMnszGzJHC2Z2dJUu59rNL0xeoomOU-TiNpHcr2pg_9_4BfScW_N</recordid><startdate>202106</startdate><enddate>202106</enddate><creator>Nowacka, Marta</creator><creator>Sterzynska, Karolina</creator><creator>Andrzejewska, Malgorzata</creator><creator>Nowicki, Michal</creator><creator>Januchowski, Radoslaw</creator><general>Elsevier Masson SAS</general><scope>6I.</scope><scope>AAFTH</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202106</creationdate><title>Drug resistance evaluation in novel 3D in vitro model</title><author>Nowacka, Marta ; Sterzynska, Karolina ; Andrzejewska, Malgorzata ; Nowicki, Michal ; Januchowski, Radoslaw</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-9d85b312e17689520538ccb5e32cce61c1fa3735d348f98577ab318cc83636593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Drug resistance</topic><topic>Ovarian cancer</topic><topic>Spheroids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nowacka, Marta</creatorcontrib><creatorcontrib>Sterzynska, Karolina</creatorcontrib><creatorcontrib>Andrzejewska, Malgorzata</creatorcontrib><creatorcontrib>Nowicki, Michal</creatorcontrib><creatorcontrib>Januchowski, Radoslaw</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedicine &amp; pharmacotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nowacka, Marta</au><au>Sterzynska, Karolina</au><au>Andrzejewska, Malgorzata</au><au>Nowicki, Michal</au><au>Januchowski, Radoslaw</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Drug resistance evaluation in novel 3D in vitro model</atitle><jtitle>Biomedicine &amp; pharmacotherapy</jtitle><date>2021-06</date><risdate>2021</risdate><volume>138</volume><spage>111536</spage><epage>111536</epage><pages>111536-111536</pages><artnum>111536</artnum><issn>0753-3322</issn><eissn>1950-6007</eissn><abstract>Ovarian cancer rates the highest mortality among all gynecological malignancies. The main reason for high mortality is the development of drug resistance. It can be related to changes in the expression of many drug resistance genes as well as expression of extracellular matrix proteins and cell density in the tumor. We developed a simple two-dimensional and three-dimensional model of drug sensitive A2780 and resistant to cisplatin and paclitaxel variants of ovarian cancer cell line. Using MTT assay, we compared drug resistance in two-dimensional and three-dimensional cell culture conditions. Real-time polymerase chain reaction analysis was used to compare the expression of drug resistance genes. The expression of proteins in spheroids was determined by immunohistochemistry. We observed a moderate increase in cisplatin resistance and a significant increase in paclitaxel resistance between two-dimensional and three-dimensional cell culture conditions. Our findings show that changes in the expression of drug resistance genes may play a crucial role in the drug resistance of cancer cells in traditional cell culture. On the other hand, the drug resistance in spheroids may result from different mechanisms such as cell density in the spheroid, extracellular matrix proteins expression and drug capacity to diffuse into the spheroid. [Display omitted] •Resistance to cisplatin and paclitaxel in two-dimensional and three-dimensional cell culture conditions was examined.•In two-dimensional cell culture, changes in the genes expression might be the pivotal mechanism leading to drug resistance.•In three-dimensional cell culture, tissue-specific mechanisms might be crucial in drug resistance development.</abstract><pub>Elsevier Masson SAS</pub><doi>10.1016/j.biopha.2021.111536</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0753-3322
ispartof Biomedicine & pharmacotherapy, 2021-06, Vol.138, p.111536-111536, Article 111536
issn 0753-3322
1950-6007
language eng
recordid cdi_proquest_miscellaneous_2555642129
source Elsevier:Jisc Collections:Elsevier Read and Publish Agreement 2022-2024:Freedom Collection (Reading list)
subjects Drug resistance
Ovarian cancer
Spheroids
title Drug resistance evaluation in novel 3D in vitro model
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T23%3A35%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Drug%20resistance%20evaluation%20in%20novel%203D%20in%20vitro%20model&rft.jtitle=Biomedicine%20&%20pharmacotherapy&rft.au=Nowacka,%20Marta&rft.date=2021-06&rft.volume=138&rft.spage=111536&rft.epage=111536&rft.pages=111536-111536&rft.artnum=111536&rft.issn=0753-3322&rft.eissn=1950-6007&rft_id=info:doi/10.1016/j.biopha.2021.111536&rft_dat=%3Cproquest_cross%3E2555642129%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c385t-9d85b312e17689520538ccb5e32cce61c1fa3735d348f98577ab318cc83636593%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2555642129&rft_id=info:pmid/&rfr_iscdi=true