Notch1 signaling enhances collagen expression and fibrosis in mouse uterus

• Published in: BioFactors (Oxford) 2021-09, Vol.47 (5), p.852-864
• Main Authors: Xu, Qi‐Xin, Zhang, Wang‐Qing, Liu, Xiao‐Zheng, Yan, Wan‐Kun, Lu, Lei, Song, Shan‐Shan, Wei, Shu‐Wen, Liu, Ying‐Nan, Kang, Jin‐Wen, Su, Ren‐Wei
• Format: Article
• Language: English
• Subjects: Animals; collagen; Collagen - genetics; Collagen - metabolism; Disease Models, Animal; extracellular matrix; Female; Fibrosis; IUA model; Mice; Mice, Transgenic; Notch signaling; Receptor, Notch1 - genetics; Receptor, Notch1 - metabolism; Signal Transduction - genetics; uterine fibrosis; Uterus - metabolism; Uterus - pathology
• ISSN: 0951-6433; 1872-8081
• DOI: 10.1002/biof.1771

Fibrosis is a pathological process characterized by abnormal activation of fibroblasts with increased synthesis of extracellular matrix components, including collagens. It may lead to loss of proper tissue architecture and organ function in clinical diseases such as systemic sclerosis and liver fibrosis. Excess accumulation of collagens is considered the primary indicator of fibrosis. Notch signaling has been reported to be involved in the fibrosis of many different organs, including the liver. Our previous study showed that the uterine‐specific over‐activation of canonical Notch1 signaling in the mouse uterus (Pgrcre/+ Rosa26N1ICD/+, OEx) results in complete infertility as a consequence of multiple developmental and physiological defects, together with increased collagen accumulation evidenced by Masson's staining. In this study, we further detected expressions of all 44 collagen genes in these Notch1 gain‐of‐function transgenic mice and found that 18 collagens have been largely affected. In another aspect, using an intrauterine adhesion model (IUA), we mimicked fibrosis in the mouse uterine. The results suggested that Notch receptors were upregulated only 3 days after induction, and most of the fibril‐forming collagen began to upregulate 6 days after the surgery. Furthermore, when induced IUA in the N1ICD‐OEx mice, the expression of collagens and fibrosis levels were significantly enhanced. At last, as a Notch signaling inhibitor, the γ‐secretase inhibitor N‐[N‐(3,5‐difl uorophenacetyl)‐L‐alanyl]‐S‐phenylglycine t‐butyl ester (DAPT) pretreatment could alleviate the expression of collagens and the symptoms of fibrosis. These results demonstrate that Notch signaling may play a role in upregulating collagens expression in endometrial fibrosis and might be a potential target of fibrosis therapy in the endometrium.