AtPng1 knockout mutant of Arabidopsis thaliana shows a juvenile phenotype, morpho-functional changes, altered stress response and cell wall modifications

In order to ascertain the role of plant transglutaminases (TGase) in growth and abiotic stress response, the AtPng1 knock out (KO) line of A. thaliana has been analyzed during plant development and under heat and wound stress. Comparing wild type (WT) and KO lines a 58-kDa band was immunodetected by...

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Bibliographic Details
Published in:Plant physiology and biochemistry 2021-10, Vol.167, p.11-21
Main Authors: Serafini-Fracassini, D., Della Mea, M., Parrotta, L., Faleri, C., Cai, G., Del Duca, S., Aloisi, I.
Format: Article
Language:English
Subjects:
A. thaliana
Abiotic stress
Arabidopsis - genetics
Arabidopsis Proteins - genetics
AtPng1p
Cell Wall
Chloroplasts
Differentiation
Gene Knockout Techniques
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase - genetics
Phenotype
Plant Development
Polyamines
Transglutaminase
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Summary:In order to ascertain the role of plant transglutaminases (TGase) in growth and abiotic stress response, the AtPng1 knock out (KO) line of A. thaliana has been analyzed during plant development and under heat and wound stress. Comparing wild type (WT) and KO lines a 58-kDa band was immunodetected by anti-AtPng1p antibody in the cell wall and chloroplasts only in the WT line. A residual TGase activity, not showing correlation with development nor stress response, was still present in the KO line. The KO line was less developed, with a juvenile phenotype characterized by fewer, smaller and less differentiated cells. Chloroplast TGase activity was insensitive to mutation. Data on stressed plants showed that (i) KO plants under heat stress were more juvenile compared to WT, (ii) different responses between WT and KO lines after wounding took place. TGase activity was not completely absent in the KO line, presenting high activity in the plastidial fraction. In general, the mutation affected A. thaliana growth and development, causing less differentiated cytological and anatomical features. •AtPng1 knock out of A. thaliana is less developed, and characterized by less differentiated cells.•KO plants under heat stress are more juvenile compared to WT.•Different responses between WT and KO lines after wounding take place.
ISSN:0981-9428
1873-2690
DOI:10.1016/j.plaphy.2021.07.024