Schumannella soli sp. nov., a novel actinomycete isolated from mangrove soil by in situ cultivation

• Published in: Antonie van Leeuwenhoek 2021-10, Vol.114 (10), p.1657-1667
• Main Authors: Li, Feina, Lu, Qinpei, Liao, Shuilin, Tuo, Li, Liu, Shaowei, Yang, Qin, Shen, Adong, Sun, Chenghang
• Format: Article
• Language: English
• Subjects: Amino acids; Bioactive compounds; Biomedical and Life Sciences; Cultivation; Cultivation techniques; Deoxyribonucleic acid; DNA; Gene amplification; Gene clusters; Gene sequencing; Genetic testing; Genomes; Genomic analysis; Hybridization; Laboratories; Life Sciences; Medical Microbiology; Metabolites; Microbiology; Microorganisms; Nature reserves; New species; Nucleotides; Original Paper; Osmoprotectants; Pediatrics; Phylogenetics; Phylogeny; Plant Sciences; Proteomes; Respiratory diseases; rRNA 16S; Secondary metabolites; Soil Science & Conservation
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-021-01631-6

A novel actinobacterial strain, designated 10F1D-1 T , was isolated from soil sample collected from Futian mangrove nature reserve, China using of the in situ cultivation technique. Preliminary analysis based on the 16S rRNA gene sequence revealed that strain 10F1D-1 T was the member of genus Schumannella with sharing highest sequence similarity (99.7%) to Schumannella luteola DSM 23141 T . Phylogenetic analyses based on 16S rRNA gene sequences and core proteome consistently exhibited that strain 10F1D-1 T formed a monophyletic clade with Schumannella luteola DSM 23141 T . Comparative genomic analyses clearly separated strain 10F1D-1 T from the only species of the genus Schumannella based on average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA-DNA hybridization (dDDH) values below the thresholds for species delineation. The genome of strain 10F1D-1 T contains the biosynthetic gene clusters for osmoprotectants to adapt to the salt environment of mangrove. Strain 10F1D-1 T also contains the biosynthetic gene clusters for bioactive compounds as secondary metabolites. On the basis of the polyphasic analysis, strain 10F1D-1 T is considered to represent a novel species of the genus Schumannella , for which the name Schumannella soli sp. nov. (type strain 10F1D-1 T  = CGMCC1.16699 T  = JCM 33146 T ) is proposed.