Influence of different warming temperatures on the vitrified testicular fragments from pre‐pubertal cat

Testicular vitrification is an alternative to preserve the genetic material of pre‐pubertal animals. However, there are few studies on post‐vitrification warming. Hence, the aim was to compare the influence of different warming temperatures on vitrified testicular fragments from pre‐pubertal cats. T...

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Bibliographic Details
Published in:Reproduction in domestic animals 2021-10, Vol.56 (10), p.1342-1348
Main Authors: Fernandes, Juliana de Souza, Tabosa, Beatriz Evaristo de Almeida, Brito, Bruna Farias, Silva, Herlon Victor Rodrigues, Lima, David Baruc Cruvinel, Silva, Lúcia Daniel Machado da
Format: Article
Language:English
Subjects:
Animals
Cell junctions
conservation
cryoprotectant
Epithelium
feline
Fragments
Glycerol
Histology
Lamina propria
Maintenance
Mitochondria
Morphology
Morphometry
Reduction
Rhodamine
rhodamine 123
Standard error
Testes
testicle
Tubules
Vitrification
warm‐up
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Summary:Testicular vitrification is an alternative to preserve the genetic material of pre‐pubertal animals. However, there are few studies on post‐vitrification warming. Hence, the aim was to compare the influence of different warming temperatures on vitrified testicular fragments from pre‐pubertal cats. The testicles were fragmented and divided into a control group (non‐vitrified) and vitrified, using an association between dimethylsulphoxide and glycerol. The vitrified fragments were warmed at 50, 55 and 60°C/5 s. Morphological and morphometric evaluations were carried out using classical histology. Afterwards, the mitochondrial activity was evaluated using Rhodamine 123. The data were expressed in mean and standard error. The differences were considered significant when p 
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.13997