A novel p -dimethylaminophenylether-based fluorescent probe for the detection of native ONOO − in cells and zebrafish

Peroxynitrite (ONOO − ) is a highly reactive substance, and plays an essential part in maintaining cellular homeostasis. It is crucial to monitor the ONOO − level in cells in normal and abnormal states. We introduced a p -dimethylaminophenylether-based fluorescent probe PDPE-PN, which could be synth...

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Bibliographic Details
Published in:Analyst (London) 2021-09, Vol.146 (17), p.5264-5270
Main Authors: Sheng, Wenlong, Wang, Kun, Gao, Na, Wang, Lizhen, Wang, Rongchun, Zhang, Xuanming, Chen, Xiqiang, Zhang, Yun, Zhu, Baocun, Liu, Kechun
Format: Article
Language:English
Subjects:
Fluorescent indicators
Homeostasis
Macrophages
Toxicity
Zebrafish
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Summary:Peroxynitrite (ONOO − ) is a highly reactive substance, and plays an essential part in maintaining cellular homeostasis. It is crucial to monitor the ONOO − level in cells in normal and abnormal states. We introduced a p -dimethylaminophenylether-based fluorescent probe PDPE-PN, which could be synthesized readily. The new probe had prominent sensitivity and specificity, and a fast response towards ONOO − . The spectral performance of probe PDPE-PN was outstanding and the limit of detection was 69 nM. Probe PDPE-PN with low toxicity was applied to detect endogenous/exogenous ONOO − in RAW 264.7 macrophages and zebrafish. Importantly, successful application of the new receptor opens up new ideas for the design of ONOO − probes.
ISSN:0003-2654
1364-5528
DOI:10.1039/d1an00608h