What's in the BAGs? Intrinsic disorder angle of the multifunctionality of the members of a family of chaperone regulators

• Published in: Journal of cellular biochemistry 2022-01, Vol.123 (1), p.22-42
• Main Authors: Marzullo, Liberato, Turco, Maria C., Uversky, Vladimir N.
• Format: Article
• Language: English
• Subjects: Adaptor Proteins, Signal Transducing - chemistry; Adaptor Proteins, Signal Transducing - metabolism; Alternative Splicing; Apoptosis Regulatory Proteins - chemistry; Apoptosis Regulatory Proteins - metabolism; BAG proteins; Binding; Humans; intrinsically disordered protein; Intrinsically Disordered Proteins - chemistry; Intrinsically Disordered Proteins - metabolism; Isoforms; Molecular Chaperones - chemistry; Molecular Chaperones - metabolism; molecular recognition feature; Pathogenesis; posttranslational modifications; Protein Binding; Protein Isoforms - metabolism; Protein Splicing; Protein structure; Protein Structure, Tertiary; protein structure‐function continuum; Proteins; protein‐protein interaction; proteoform; Signal Transduction; Splicing; Structure-function relationships; Translation initiation
• ISSN: 0730-2312; 1097-4644
• DOI: 10.1002/jcb.30123

In humans, the family of Bcl‐2 associated athanogene (BAG) proteins includes six members characterized by exceptional multifunctionality and engagement in the pathogenesis of various diseases. All of them are capable of interacting with a multitude of often unrelated binding partners. Such binding promiscuity and related functional and pathological multifacetedness cannot be explained or understood within the frames of the classical “one protein–one structure–one function” model, which also fails to explain the presence of multiple isoforms generated for BAG proteins by alternative splicing or alternative translation initiation and their extensive posttranslational modifications. However, all these mysteries can be solved by taking into account the intrinsic disorder phenomenon. In fact, high binding promiscuity and potential to participate in a broad spectrum of interactions with multiple binding partners, as well as a capability to be multifunctional and multipathogenic, are some of the characteristic features of intrinsically disordered proteins and intrinsically disordered protein regions. Such functional proteins or protein regions lacking unique tertiary structures constitute a cornerstone of the protein structure‐function continuum concept. The aim of this paper is to provide an overview of the functional roles of human BAG proteins from the perspective of protein intrinsic disorder which will provide a means for understanding their binding promiscuity, multifunctionality, and relation to the pathogenesis of various diseases.