O-mannosyltransferase MaPmt2 contributes to stress tolerance, cell wall integrity and virulence in Metarhizium acridum

• Published in: Journal of invertebrate pathology 2021-09, Vol.184, p.107649-107649, Article 107649
• Main Authors: Wen, Zhiqiong, Tian, Huiting, Xia, Yuxian, Jin, Kai
• Format: Article
• Language: English
• Subjects: Cell wall integrity; MaPmt2; Metarhizium acridum; O-mannosyltransferase; Virulence
• ISSN: 0022-2011; 1096-0805
• DOI: 10.1016/j.jip.2021.107649

[Display omitted] •Knockdown of MaPmt2 affected cell wall integrity and components.•Knockdown of MaPmt2 decreased fungal conidial yield and multi-stress tolerances.•Knockdown of MaPmt2 reduced fungal virulence mainly by reducing the turgor pressure in appressorium. As a conserved post-translational modification, O-mannosyltransferase families play important roles in many cellular processes. Three subfamilies (MaPmt1, MaPmt2 and MaPmt4) are grouped in Metarhizium acridum according to sequence homology. The functions of MaPmt1 and MaPmt4 have been characterized in M. acridum previously. In this study, the functions of another member belonging to the Pmt2 subfamily, MaPmt2, were identified through RNAi strategy. The three MaPmt2 knockdown mutants showed dramatically decreased expression of MaPmt2. Phenotypic analyses showed that the mutants exhibited decreased tolerances to wet-heat, UV-B irradiation and cell wall perturbing chemicals. Further studies revealed that the mutants presented thinner cell walls observed by transmission electron microscope combined with changed cell wall components. Besides, knockdown of MaPmt2 decelerated conidial germination and decreased conidial yield. Compared with the wild-type strain, the MaPmt2 knockdown mutants caused impaired virulence only by topical inoculation. Results illustrated that the decreased virulence by inoculation could result from the delayed conidial germination on locust wings, reduced appressorium formation, as well as reduced turgor pressure in MaPmt2 knockdown mutants.