Preclinical mouse model of optical coherence tomography for subcortical brain imaging without dissection

• Published in: Journal of biophotonics 2021-11, Vol.14 (11), p.e202100143-n/a
• Main Authors: Lee, Jae Meen, Han, Inho, Nam, Kyoung Hyup, Kim, Dong Hwan, Song, Seunghwan, Park, Heejeong, Kim, Hongki, Kim, Minkyu, Choi, Jeonghun, Lee, Jae Il
• Format: Article
• Language: English
• Subjects: Animals; Brain; Brain - diagnostic imaging; Dissection; Hematoma; Hemorrhage; intracerebral hematoma; Lesions; Medical imaging; Mice; Mouse devices; Neuroimaging; neurosurgery; Optical Coherence Tomography; Tomography; Tomography, Optical Coherence
• ISSN: 1864-063X; 1864-0648
• DOI: 10.1002/jbio.202100143

The purpose of this study was to investigate the feasibility of using optical coherence tomography (OCT) to identify internal brain lesions, specifically intracerebral hemorrhage, without dissection. Mice with artificially injected brain hematomas were used to test the OCT system, and the recorded images were compared with microscopic images of the same mouse brains after hematoxylin and eosin staining. The intracranial structures surrounding the hematomas were clearly visualized by the OCT system without dissection. These images reflect the ability of OCT to determine the extent of a lesion in several planes. OCT is a useful technology, and these findings could be used as a starting point for future research in intraoperative imaging. The purpose of this study was to investigate the feasibility of using optical coherence tomography (OCT) to identify internal mice brain lesions, injected hemorrhage, in several planes without dissection. After our experiments, OCT is a useful technology, and these findings could be used as a starting point for future research in intraoperative imaging.