Eco‐physiological portrait of a novel Pseudomonas sp. CSV86: an ideal host/candidate for metabolic engineering and bioremediation

• Published in: Environmental microbiology 2022-06, Vol.24 (6), p.2797-2816
• Main Authors: Phale, Prashant S., Mohapatra, Balaram, Malhotra, Harshit, Shah, Bhavik A.
• Format: Article
• Language: English
• Subjects: Affinity; Aromatic compounds; Benzoates; Benzoic acid; Bioremediation; Carbon sources; Clusters; Gene transfer; Genomes; Genomic islands; Glucokinase; Glucose; Glucose metabolism; Horizontal transfer; Metabolic engineering; Metabolism; Metabolites; Naphthalene; Organic acids; Oxidative metabolism; Pathogenicity; Pathogens; Phenotypes; Phenylpropanoids; Physiological effects; Physiology; Pseudomonas; Virulence; Virulence factors; Xenobiotics
• ISSN: 1462-2912; 1462-2920
• DOI: 10.1111/1462-2920.15694

Summary Pseudomonas sp. CSV86, an Indian soil isolate, degrades wide range of aromatic compounds like naphthalene, benzoate and phenylpropanoids, amongst others. Isolate displays the unique and novel property of preferential utilization of aromatics over glucose and co‐metabolizes them with organic acids. Interestingly, as compared to other Pseudomonads, strain CSV86 harbours only high‐affinity glucokinase pathway (and absence of low‐affinity oxidative route) for glucose metabolism. Such lack of gluconate loop might be responsible for the novel phenotype of preferential utilization of aromatics. The genome analysis and comparative functional mining indicated a large genome (6.79 Mb) with significant enrichment of regulators, transporters as well as presence of various secondary metabolite production clusters, suggesting its eco‐physiological and metabolic versatility. Strain harbours various integrative conjugative elements (ICEs) and genomic islands, probably acquired through horizontal gene transfer events, leading to genome mosaicity and plasticity. Naphthalene degradation genes are arranged as regulonic clusters and found to be part of ICECSV86nah. Various eco‐physiological properties and absence of major pathogenicity and virulence factors (risk group‐1) in CSV86 suggest it to be an ideal candidate for bioremediation. Further, strain can serve as an ideal chassis for metabolic engineering to degrade various xenobiotics preferentially over simple carbon sources for efficient remediation.