SigB regulates stress resistance, glucose starvation, MnSOD production, biofilm formation, and root colonization in Bacillus cereus 905

• Published in: Applied microbiology and biotechnology 2021-08, Vol.105 (14-15), p.5943-5957
• Main Authors: Gao, Tantan, Li, Yan, Chai, Yunrong, Wang, Qi, Ding, Mingzheng
• Format: Article
• Language: English
• Subjects: Analysis; Applied Genetics and Molecular Biotechnology; Bacillus cereus; Biofilms; Biomedical and Life Sciences; Biotechnology; Carbon sources; Colonization; Environmental conditions; Environmental stress; Ethanol; Genes; Genetic aspects; Genetic transcription; Glucose; Glucose metabolism; Identification and classification; Life Sciences; Manganese; Microbial Genetics and Genomics; Microbiology; Mutants; Oxidative stress; Paraquat; Properties; Rhizosphere; Roots; SigB gene; Sigma factor; Starvation; Superoxide dismutase; Survival; Transcription; Wheat
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-021-11402-y

Bacillus cereus 905, originally isolated from wheat rhizosphere, exhibits strong colonization ability on wheat roots. Our previous studies showed that root colonization is contributed by the ability of the bacterium to efficiently utilize carbon sources and form biofilms and that the sodA2 gene-encoded manganese-containing superoxide dismutase (MnSOD2) plays an indispensable role in the survival of B. cereus 905 in the wheat rhizosphere. In this investigation, we further demonstrated that the ability of B. cereus 905 to resist adverse environmental conditions is partially attributed to activation of the alternative sigma factor σ B , encoded by the sigB gene. The sigB mutant experienced a dramatic reduction in survival when cells were exposed to ethanol, acid, heat, and oxidative stress or under glucose starvation. Analysis of the sodA2 gene transcription revealed a partial, σ B -dependent induction of the gene during glucose starvation or when treated with paraquat. In addition, the sigB mutant displayed a defect in biofilm formation under stress conditions. Finally, results from the root colonization assay indicated that sigB and sodA2 collectively contribute to B. cereus 905 colonization on wheat roots. Our study suggests a diverse role of SigB in rhizosphere survival and root colonization of B. cereus 905 under stress conditions. Key points • SigB confers resistance to environmental stresses in B. cereus 905. • SigB plays a positive role in glucose utilization and biofilm formation in B. cereus. • SigB and SodA2 collectively contribute to colonization on wheat roots by B. cereus.