Molecular cloning, sequence, and expression patterns of DNA damage induced transcript 3 (DDIT3) gene in female yaks (Bos grunniens)

Endoplasmic reticulum stress (ERS) plays an important role in regulating the reproductive process of female mammals, mainly involved in follicular atresia and corpus luteum regression. DNA damage induced transcript 3 (DDIT3) is a marker gene of ERS. The objectives of the present study were to clone...

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Bibliographic Details
Published in:Animal biotechnology 2023-04, Vol.34 (2), p.280-287
Main Authors: Wu, Jian-fei, Liu, Yu, Zi, Xiang-dong, Li, Heng, Lu, Jian-yuan, Jing, Tian
Format: Article
Language:English
Subjects:
Animals
Bos grunniens
Cattle
Cloning
Cloning, Molecular
Complementary DNA
Corpus luteum
Damage patterns
DDIT3
Deoxyribonucleic acid
DNA
DNA damage
Endoplasmic reticulum
Female
Females
Fetuses
Follicular Atresia
Gametocytes
Gene expression
Granulosa cells
Horses
Mammals
Metaphase
Nucleotide sequence
oocyte
Oocytes
Ovaries
ovary
Ovary - metabolism
Oviduct
Polymerase chain reaction
Pregnancy
Real-Time Polymerase Chain Reaction
RNA-directed DNA polymerase
Uterus
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Summary:Endoplasmic reticulum stress (ERS) plays an important role in regulating the reproductive process of female mammals, mainly involved in follicular atresia and corpus luteum regression. DNA damage induced transcript 3 (DDIT3) is a marker gene of ERS. The objectives of the present study were to clone and analyze the sequence and tissue expression characteristics of DDIT3 gene in female yaks. By reverse transcriptase-polymerase chain reaction (RT-PCR) strategy, we obtained full-length 507-bp DDIT3-cDNA, encoding for 168-aa protein. Yak DDIT3 exhibited highest and least identity with that of bison and horse, respectively. Real-time PCR analyses revealed that the expression level of DDIT3 gene in ovary was higher than that in heart, liver, kidney, spleen, lung, uterus and oviduct (p 
ISSN:1049-5398
1532-2378
DOI:10.1080/10495398.2021.1957686