Comparison of two routes of administration of a cationic liposome formulation for a prophylactic DC vaccination in a murine melanoma model

• Published in: International immunopharmacology 2021-09, Vol.98, p.107833-107833, Article 107833
• Main Authors: Yazdani, Mona, Nikpoor, Amin Reza, Gholizadeh, Zahra, Mohamadian Roshan, Nema, Seifalian, Alexander, Jaafari, Mahmoud Reza, Badiee, Ali
• Format: Article
• Language: English
• Subjects: Animals; Antibodies; Antigen Presentation; Antigens; Antineoplastic Agents, Immunological - pharmacology; Antineoplastic Agents, Immunological - therapeutic use; Cancer Vaccines - immunology; Cations; Combined Modality Therapy; Dendritic cells; Dendritic Cells - immunology; Dendritic Cells - transplantation; Disease Models, Animal; DOTAP; Drug Administration Routes; Glycoprotein gp100; gp100; gp100 Melanoma Antigen - metabolism; Humans; Immunization; Immunoregulation; Immunotherapy, Adoptive - methods; In vivo methods and tests; Interleukin 10; Liposome; Liposomes; Liposomes - chemical synthesis; Liposomes - metabolism; Lymphocytes; Lymphocytes T; Lymphocytes, Tumor-Infiltrating - immunology; Melanoma; Melanoma - immunology; Melanoma - therapy; Melanoma, Experimental; Mice; Mice, Inbred C57BL; PD-1 monoclonal antibody; PD-1 protein; Programmed Cell Death 1 Receptor - antagonists & inhibitors; Skin Neoplasms - immunology; Skin Neoplasms - therapy; T-Lymphocytes, Regulatory - immunology; Tumor microenvironment; Tumors; Vaccination; Vaccine; Vaccines; γ-Interferon
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2021.107833

The scheme represents the comparative study of in vivo and ex vivo DC vaccination efficacy when applied as a combination with PD-1 blockade antibody. [Display omitted] •Liposomal pulsed ex vivo DC–based vaccine in combination with PD-1 blockade significantly.•Downregulated the intratumoral expression level of inhibitory cytokines, IL-10 and TGF-β over in vivo approach.•Increased the number of PD-1 expressing TILs in TME over in vivo approach.•Improved tumor growth regression and survival rate over in vivo approach. Dendritic cell (DC) vaccination can be achieved via straight loading of vaccine into DCs ex vivo or administration to DCs in vivo. However, there is no certain consensus on which approach is preferable, and each strategy has its advantages and disadvantages, which affect the efficacy and safety of vaccines. It will also be more complicated when a vaccine delivery system is included. In this study, the efficacy of ex vivo pulsed DC-based vaccine compared with in vivo subcutaneous administration of a cationic liposomes (CLs) formulation containing gp100 antigen (gp100-CLs) was evaluated in a murine melanoma model. In combination with an anti-PD-1 antibody, the ex vivo approach of gp100-CLs yielded a significant (P