High-frequency near-infrared diode laser irradiation suppresses IL-1β-induced inflammatory cytokine expression and NF-κB signaling pathways in human primary chondrocytes

• Published in: Lasers in medical science 2022-03, Vol.37 (2), p.1193-1201
• Main Authors: Sakata, Shuzo, Kunimatsu, Ryo, Tsuka, Yuji, Nakatani, Ayaka, Gunji, Hidemi, Yanoshita, Makoto, Kado, Isamu, Ito, Shota, Putranti, Nurul Aisyah Rizky, Prasetya, Rendra Chriestedy, Hirose, Naoto, Tanimoto, Kotaro
• Format: Article
• Language: English
• Subjects: Airline security; Arthritis; Binding; Biomedical materials; Cartilage; Cartilage diseases; Cells, Cultured; Chondrocytes; Chondrocytes - pathology; Cytokines; Cytokines - metabolism; Dentistry; Deoxyribonucleic acid; DNA; Electrophoretic mobility; Enzyme-linked immunosorbent assay; Frequency analysis; Humans; I.R. radiation; IL-1β; Immunofluorescence; Inflammation; Infrared lasers; Interleukin 1; Interleukin 6; Interleukin-1beta - metabolism; Interleukin-1beta - pharmacology; Irradiation; Lasers; Lasers, Semiconductor - therapeutic use; Light therapy; Matrix metalloproteinase; Matrix metalloproteinases; Medicine; Medicine & Public Health; NF-kappa B - metabolism; NF-κB protein; Nuclear transport; Optical Devices; Optics; Original Article; Osteoarthritis; Phosphorylation; Photonics; Quantum Optics; Rheumatoid arthritis; Semiconductor lasers; Signal Transduction; Signaling; Translocation; Tumor necrosis factor-α
• ISSN: 0268-8921; 1435-604X
• DOI: 10.1007/s10103-021-03371-5

Osteoarthritis (OA) and rheumatoid arthritis (RA) are common inflammation-associated cartilage degenerative diseases. Recent studies have shown that low-level diode laser treatment can reduce inflammatory cytokine expressions in cartilage. We recently reported that high-frequency low-level diode laser irradiation attenuates matrix metalloproteinases (MMPs) expression in human primary chondrocytes. However, the molecular mechanism underlying the effect of high-frequency low-level diode laser on chondrocytes remains unclear. Therefore, we aimed to elucidate the effect of high-frequency low-level diode laser irradiation on inflammatory cytokine expression in human primary chondrocytes. Normal human articular chondrocytes were treated with recombinant interleukin-1 beta (IL-1β) for 30 min or 24 h and irradiated with a high-frequency NIR diode laser at 8 J/cm 2 . The expression of IL-1β, interleukin-6, and tumor necrosis factor-alpha was assessed using western blot analysis. To evaluate the nuclear factor-kappa B (NF-κB) signaling pathway, the phosphorylation, translocation, and DNA-binding activity of NF-κB were detected using western blot analysis, immunofluorescence analysis, electrophoretic mobility shift assay, and enzyme-linked immunosorbent assay analysis. High-frequency low-level diode laser irradiation decreased inflammatory cytokine expression in IL-1β-treated chondrocytes. Moreover, high-frequency low-level diode laser irradiation decreased the phosphorylation, nuclear translocation, and DNA-binding activity of NF-κB in the IL-1β-treated state. However, irradiation alone did not affect NF-κB activity. Thus, high-frequency low-level diode laser irradiation at 8 J/cm 2 can reduce inflammatory cytokine expressions in normal human articular chondrocytes through NF-κB regulation. These findings indicate that high-frequency low-level diode laser irradiation may reduce the expression of inflammatory cytokines in OA and RA.