Selenium inorganic speciation in beers using MSFIA-HG-AFS system after multivariate optimization

• Published in: Food chemistry 2022-01, Vol.367, p.130673-130673, Article 130673
• Main Authors: Souza, Sidnei O., Ávila, Dayara Virgínia L., Cerdà, Victor, Araujo, Rennan Geovanny O.
• Format: Article
• Language: English
• Subjects: Beer; Doehlert; MSFIA-HG-AFS; Selenium; Speciation
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2021.130673

•Experimental conditions of hydride generation were optimized through Doehlert design.•Beer samples effectively analyzed by MSFIA-HG-AFS system.•Precise, accurate and sensitivity procedure for Se and species determination is proposed.•Total inorganic Se, Se(IV) and Se(VI) concentrations were determined in beer samples.•Beer is an alternative source for incorporation of selenium in Spanish diet. In this work, the use of a multisyringe flow injection analysis coupled to hydride generation atomic fluorescence spectrometry (MSFIA-HG-AFS) for inorganic selenium chemical speciation was proposed. A Doehlert design was applied to optimize the experimental conditions for hydride generation (NaBH4 and HCl concentrations). The limits of quantification (LoQ) obtained were 0.07 µg L−1, for total inorganic Se, and 0.08 µg L−1, for Se(IV). Accuracy and precision of the proposed analytical method were evaluated through analysis of standard reference material and addition and recovery tests. The optimized method was applied to analyses of eight samples of beer, produced in Spain, obtaining concentrations for Se(IV) (