Long‐Term Observation of Locomotion of Drosophila Larvae Facilitates Feasibility of Food‐Choice Assays

Animal behavior is reflected by locomotor patterns. To decipher the underlying neural circuitry locomotion has to be monitored over often longer time periods. Here a simple adaptation is described to constrain movement of third instar Drosophila larvae to a defined area and use Frustrated total inte...

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Bibliographic Details
Published in:Advanced biology 2022-04, Vol.6 (4), p.e2100938-n/a
Main Authors: Bittern, Jonas, Praetz, Marit, Baldenius, Marie, Klämbt, Christian
Format: Article
Language:English
Subjects:
Animals
Drosophila
Feasibility Studies
FIM
Food Preferences
Larva
Locomotion
long‐term observation
third instar larva
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Summary:Animal behavior is reflected by locomotor patterns. To decipher the underlying neural circuitry locomotion has to be monitored over often longer time periods. Here a simple adaptation is described to constrain movement of third instar Drosophila larvae to a defined area and use Frustrated total internal reflection based imaging method (FIM) imaging to monitor larval movements up to 1 h. It is demonstrated that the combination of FIM imaging and long analysis periods facilitates the conduction of food choice assays and provides the means to easily quantify food preferences. Locomotion analysis is a key to decipher neuronal circuitry. Constraining movements of Drosophila larvae to an imaging arena allows monitoring of locomotion for several hours. This improvement in long‐term analysis of locomotion facilitates the analysis of food preference or even more complex behaviors like circadian rhythms.
ISSN:2701-0198
2701-0198
DOI:10.1002/adbi.202100938