Effect of valproic acid on miRNAs affecting histone deacetylase in a model of anaplastic thyroid cancer

• Published in: Molecular biology reports 2021-08, Vol.48 (8), p.6085-6091
• Main Authors: Gunel, Nur Selvi, Birden, Nihal, Kurt, Cansu Caliskan, Bagca, Bakiye Goker, Shademan, Behrouz, Sogutlu, Fatma, Ozates, Neslihan Pinar, Avci, Cigir Biray
• Format: Article
• Language: English
• Subjects: Animal Anatomy; Animal Biochemistry; Biomarkers, Tumor - genetics; Biomedical and Life Sciences; Cell Line, Tumor; Chemotherapy; Chromosome 5; Endocrine system; Gene expression; Gene Expression - genetics; Gene Expression Profiling - methods; Gene Expression Regulation, Neoplastic - genetics; Histology; Histone deacetylase; Histone Deacetylases - genetics; Histone Deacetylases - metabolism; Humans; Iodine; Life Sciences; Life span; MicroRNAs - drug effects; MicroRNAs - genetics; miRNA; Models, Biological; Morphology; Original Article; Papillary thyroid cancer; Polymerase chain reaction; Thyroid cancer; Thyroid carcinoma; Thyroid Carcinoma, Anaplastic - drug therapy; Thyroid Carcinoma, Anaplastic - genetics; Thyroid Gland - metabolism; Transcriptome - genetics; Valproic acid; Valproic Acid - metabolism; Valproic Acid - pharmacology
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-021-06616-2

Background Thyroid cancer is the most common malignant tumor of the endocrine system seen in the thyroid gland. More than 90% of thyroid cancers comprise papillary thyroid cancer (PTC) and follicular thyroid cancer (FTC). Although anaplastic thyroid carcinoma (ATC) accounts for less than 2% of thyroid cancer. But patients’ lifespan after diagnosis is about 6 months. Surgical interventions, radioactive iodine use, and chemotherapy are not sufficient in the treatment of ATC, so alternative therapies are needed. Methods and results The WST-1 assay test was performed to evaluate the anti-proliferative effects of Valproic acid (VPA). Also, the effect of VPA on miRNAs affecting histone deacetylase was determined by Quantitative RT-PCR. In the SW1736 cell line, IC50 dose for VPA was found 1.6 mg/ml. In our study, the level of oncogenic genes expression in cells treated with VPA, including miR-184, miR-222-5p, miR-124-3p, and miR-328-3p, decreased. Also, the expression of tumor inhibitory genes including miR-323-5p, miR-182-5p, miR-138-5p, miR-217, miR-15a-5p, miR-29b-3p, miR-324-5p and miR-101-5p increased significantly. Conclusions VPA can ad-just countless gene expression patterns, including microRNAs (miRNAs), by targeting histone deacetylase (HDAC). However, further studies are required for more accurate results.