Giant Y79 retinoblastoma cells contain functionally active T-type calcium channels

Retinoblastoma is the most common malignant intraocular tumor in children. Y79 human retinoblastoma cells are in vitro models of retinal tumors used for drug screening. Undifferentiated Y79 cells originate from a primitive multi-potential neuroectodermal cell and express neuronal and glial propertie...

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Bibliographic Details
Published in:Pflügers Archiv 2021-10, Vol.473 (10), p.1631-1639
Main Authors: Kim, Sooyun, Guzman, Segundo Jose, Jo, Dong Hyun, Cho, Chang Sik, Kim, Jeong Hun
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Calcium channels
Calcium channels (T-type)
Calcium Channels, T-Type - metabolism
Calcium influx
Cancer
Cell Biology
Cell culture
Cell growth
Cell Line, Tumor
Drug screening
Eye diseases
Gene Expression Regulation, Neoplastic
Giant cells
Giant Cells - metabolism
Glial cells
Human Physiology
Humans
Ion Channels
Membrane potential
Molecular Medicine
Neuronal-glial interactions
Neurosciences
Physiology
Receptors
Receptors and Transporters
Retina
Retinal Neoplasms - genetics
Retinal Neoplasms - metabolism
Retinoblastoma
Retinoblastoma - genetics
Retinoblastoma - metabolism
Tumors
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Summary:Retinoblastoma is the most common malignant intraocular tumor in children. Y79 human retinoblastoma cells are in vitro models of retinal tumors used for drug screening. Undifferentiated Y79 cells originate from a primitive multi-potential neuroectodermal cell and express neuronal and glial properties. However, the nature of cellular heterogeneity in Y79 cells is unclear because functional methods to characterize neurons or glial cells have not been employed to Y79 cells. Here, we perform patch-clamp recordings to characterize electrophysiological properties in retinoblastoma cells. We identified a population of large-sized Y79 cells (i.e., giant cells, ~ 40-µm diameter), hyperpolarized resting membrane potential (–54 mV), and low input resistance (~ 600 MΩ), indicating electrically mature cells. We also found that giant Y79 cells contain increased density of T-type calcium channels. Finally, we found that T-type calcium channels are active only in giant cells suggesting that cancer treatments aimed to prevent calcium influx in retinoblastomas should be tested in giant cells.
ISSN:0031-6768
1432-2013
DOI:10.1007/s00424-021-02612-4