The role of phenylalanine hydroxylase in lipogenesis in the oleaginous fungus Mortierella alpina

Phenylalanine hydroxylase (PAH) catalyses the irreversible hydroxylation of phenylalanine to tyrosine, which is the rate-limiting reaction in phenylalanine metabolism in animals. A variety of polyunsaturated fatty acids can be synthesized by the lipid-producing fungus , which has a wide range of ind...

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Bibliographic Details
Published in:Microbiology (Society for General Microbiology) 2021-08, Vol.167 (8)
Main Authors: Wang, Hongchao, Wang, Chunmei, Yuan, Weiwei, Chen, Haiqin, Lu, Wenwei, Zhang, Hao, Chen, Yong Q, Zhao, Jianxin, Chen, Wei
Format: Article
Language:English
Subjects:
Arachidonic Acid
Lipogenesis - genetics
Mortierella - genetics
Phenylalanine Hydroxylase - genetics
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Summary:Phenylalanine hydroxylase (PAH) catalyses the irreversible hydroxylation of phenylalanine to tyrosine, which is the rate-limiting reaction in phenylalanine metabolism in animals. A variety of polyunsaturated fatty acids can be synthesized by the lipid-producing fungus , which has a wide range of industrial applications in the production of arachidonic acid. In this study, RNA interference (RNAi) with the gene was used to explore the role of phenylalanine hydroxylation in lipid biosynthesis in . Our results indicated that knockdown decreased the transcript level by approximately 55% and attenuated cellular fatty acid biosynthesis. Furthermore, the level of NADPH, which is a critical reducing agent and the limiting factor in lipogenesis, was decreased in response to RNAi, in addition to the downregulated transcription of other genes involved in NADPH production. Our study indicates that PAH is part of an overall enzymatic and regulatory mechanism supplying NADPH required for lipogenesis in .
ISSN:1350-0872
1465-2080
DOI:10.1099/mic.0.001062