Profile for mRNA transcript abundances in the pig endometrium where inflammation was induced by Escherichia coli

• Published in: Animal reproduction science 2021-09, Vol.232, p.106824-106824, Article 106824
• Main Authors: Jana, Barbara, Kaczmarek, Monika M., Romaniewicz, Marta, Brzozowska, Marta
• Format: Article
• Language: English
• Subjects: Animals; Computational Biology; Endometritis - microbiology; Endometritis - veterinary; Endometrium; Endometrium - physiology; Escherichia coli; Escherichia coli Infections - metabolism; Escherichia coli Infections - veterinary; Female; Gene Expression Regulation; Inflammation - metabolism; Inflammation - microbiology; Inflammatory process; Pig; Protein Array Analysis; Reproducibility of Results; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sus scrofa; Swine - physiology; Transcriptome profiling
• ISSN: 0378-4320; 1873-2232
• DOI: 10.1016/j.anireprosci.2021.106824

•Inflammation up-regulates S100A9, SLC11A1, CCL15, CCL3L3, CCR1 genes in endometrium.•Inflammation down-regulates IL24, FGG, SST, CXCL16, CREB genes in endometrium.•The most altered genes are involved in the glucocorticoid receptor signaling pathway.•LPS is a top upstream regulator for differentially expressed genes. Uterine inflammation is a common reproductive disorder in domestic animals, leading to disturbances in many reproductive processes and economic losses. More information on inflammatory pathways, however, is needed to understand mechanisms of uterine inflammation. The aim of the study was to investigate transcriptomic profiles of the pig endometrium affected by inflammation. On day 3 of the estrous cycle (day 0 = initial day of study), saline or Escherichia coli suspension were injected into uterine horns. In endometrial tissues collected 8 days later, microarray analysis results indicated there were 189 differentially abundant mRNA transcripts (DEGs, 95 in relatively greater and 94 in lesser abundance) after saline injections compared with samples where there was severe acute inflammation. Relative abundance of mRNA transcripts for proteins assigned to inflammatory response, movement of phagocytes, quantity of phagocytes, leukocyte migration and adhesion of immune cells and many other functions related to inflammation were different in the Escherichia coli-treated endometrium than in samples from gilts treated with saline. Among others, S100A9, SLC11A1, CCL15, CCL3L3, CCR1, CD48, CD163, THBS1, KIT, ITGB3, JAK3 and NFKB2 mRNA transcripts were in relatively greater abundance and there were those in relatively lesser abundance including IL24, FGG, SST, CXCL16 and CREB. In this study, for the first time, there was detection of alterations in the transcriptome of the inflamed pig endometrium which may be an important finding for maintaining uterine homeostasis and functions. Results form the basis for future studies focusing on regulation of uterine inflammation in animals and women.