Simultaneously deleting ADH2 and THI3 genes of Saccharomyces cerevisiae for reducing the yield of acetaldehyde and fusel alcohols

• Published in: FEMS microbiology letters 2021-08, Vol.368 (15), p.1
• Main Authors: Wu, Liang, Wen, Yongdi, Chen, Wenying, Yan, Tongshuai, Tian, Xiaofei, Zhou, Shishui
• Format: Article
• Language: English
• Subjects: Acetaldehyde; Acetaldehyde - metabolism; Alcohol; Alcohol dehydrogenase; Alcohol Dehydrogenase - genetics; Alcoholic beverages; Alcohols - metabolism; Analysis; Brewing; Butanol; Carrier Proteins - genetics; Decarboxylases; Deletion mutant; Diploids; Ethanol; Fermentation; Flavor; Fungi; Gene Deletion; Gene mutations; Genes; Genetic aspects; Identification and classification; Industrial Microbiology - methods; Isopentyl alcohol; Methods; Microbiology; Mutants; Saccharomyces; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Yeast
• ISSN: 1574-6968; 0378-1097; 1574-6968
• DOI: 10.1093/femsle/fnab094

ABSTRACT The reduced yields of acetaldehyde and fusel alcohols through fermentation by Saccharomyces cerevisiae is of significance for the improvement of the flavor and health of alcoholic beverages. In this study, the ADH2 (encode alcohol dehydrogenase) and THI3 (encode decarboxylase) genes of the industrial diploid strain S. cerevisiae XF1 were deleted. Results showed that single-gene-deletion mutants by separate gene deletion of ADH2 or THI3 led to a reduced production of the acetaldehyde or fusel alcohols, respectively. In the meantime, the double-gene-deletion mutant S. cerevisiae XF1-AT was constructed by deleting the ADH2 and THI3 simultaneously. An equivalent level of the ethanol production by the S. cerevisiae XF1-AT could be achieved but with the yields of acetaldehyde, isoamyl alcohol and iso-butanol reduced by 42.09%, 15.65% and 20.16%, respectively. In addition, there was no interaction between the ADH2 deletion and THI3 deletion in reducing the production of acetaldehyde and fusel alcohols. The engineered S. cerevisiae XF1-AT provided a new strategy to alcoholic beverages brewing industry for reducing the production of acetaldehyde as well as the fusel alcohols. The acetaldehyde and fusel alcohols level were decreased by simultaneously deleting ADH2 and THI3 genes of S. cerevisiae in order to improve the quality of alcoholic beverages.