Plant compounds and nonsteroidal anti-inflammatory drugs interfere with quorum sensing in Chromobacterium violaceum

Chromobacterium violaceum is a Gram-negative, saprophytic bacterium that can infect humans and its virulence may be regulated by quorum sensing via N -acyl homoserine lactones. A virtual screening study with plant compounds and nonsteroidal anti-inflammatory drugs for inhibition of C. violaceum quor...

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Bibliographic Details
Published in:Archives of microbiology 2021-11, Vol.203 (9), p.5491-5507
Main Authors: Vargas, Erika Lorena Giraldo, de Almeida, Felipe Alves, de Freitas, Leonardo Luiz, Pinto, Uelinton Manoel, Vanetti, Maria Cristina Dantas
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Anti-inflammatory agents
Anti-Inflammatory Agents - pharmacology
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cell Biology
Chromobacterium - drug effects
Chromobacterium violaceum
Dipyrone
Ecology
Homoserine lactones
Inflammation
Lactones
Life Sciences
Microbial Ecology
Microbiology
Molecular docking
Molecular Docking Simulation
Nonsteroidal anti-inflammatory drugs
Original Paper
Palmitic acid
Phenotypes
Phytol
Plant Extracts - pharmacology
Quorum Sensing
Virulence
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Summary:Chromobacterium violaceum is a Gram-negative, saprophytic bacterium that can infect humans and its virulence may be regulated by quorum sensing via N -acyl homoserine lactones. A virtual screening study with plant compounds and nonsteroidal anti-inflammatory drugs for inhibition of C. violaceum quorum sensing system has been performed. In vitro evaluation was done to validate the in silico results. Molecular docking showed that phytol, margaric acid, palmitic acid, dipyrone, ketoprofen, and phenylbutazone bound to structures of CviR proteins of different C. violaceum strains. Phytol presented higher binding affinities than AHLs and furanones, recognized inducers, and inhibitors of quorum sensing, respectively. When tested in vitro, phytol at a non-inhibitory concentration was the most efficient tested compound to reduce phenotypes regulated by quorum sensing. The results indicate that in silico compound prospection to inhibit quorum sensing may be a good tool for finding alternative lead molecules.
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-021-02518-w