Impact of macromolecular crowding on the mesomorphic behavior of lipid self-assemblies

• Published in: Biochimica et biophysica acta. Biomembranes 2021-12, Vol.1863 (12), p.183728-183728, Article 183728
• Main Authors: Mangiarotti, Agustín, Bagatolli, Luis A.
• Format: Article
• Language: English
• Subjects: 2-Naphthylamine - analogs & derivatives; 2-Naphthylamine - chemistry; Gelatin - chemistry; Glycerides - chemistry; Immunoglobulin G - chemistry; Laurates - chemistry; LAURDAN fluorescence; Lipid membrane hydration; Lipid polymorphism; Lipids - chemistry; Macromolecular crowding; Molecular Conformation; PEG; Phosphatidylcholines - chemistry; Polyethylene Glycols - chemistry; Polymers - chemistry; Protein secondary structure; Serum Albumin, Human - chemistry; Water - chemistry
• ISSN: 0005-2736; 1879-2642
• DOI: 10.1016/j.bbamem.2021.183728

Using LAURDAN fluorescence we observed that water dynamics measured at the interface of DOPC bilayers can be differentially regulated by the presence of crowded suspensions of different proteins (HSA, IgG, Gelatin) and PEG, under conditions where the polymers are not in direct molecular contact with the lipid interface. Specifically, we found that the decrease in water dipolar relaxation at the membrane interface correlates with an increased fraction of randomly oriented (or random coil) configurations in the polymers, as Gelatin > PEG > IgG > HSA. By using the same experimental strategy, we also demonstrated that structural transitions from globular to extended conformations in proteins can induce transitions between lamellar and non-lamellar phases in mixtures of DOPC and monoolein. Independent experiments using Raman spectroscopy showed that aqueous suspensions of polymers exhibiting high proportions of randomly oriented conformations display increased fractions of tetracoordinated water, a configuration that is dominant in ice. This indicates a greater capacity of this type of structure for polarizing water and consequently reducing its chemical activity. This effect is in line with one of the tenets of the Association Induction Hypothesis, which predicts a long-range dynamic structuring of water molecules via their interactions with proteins (or other polymers) showing extended conformations. Overall, our results suggest a crucial role of water in promoting couplings between structural changes in macromolecules and supramolecular arrangements of lipids. This mechanism may be of relevance to cell structure/function when the crowded nature of the intracellular milieu is considered. [Display omitted] •Macromolecular crowding can modify membrane hydration.•Distinct protein secondary structures differentially modulate water activity.•Lipid polymorphism can be induced by proteins existing in extended configurations.