A nanohybrid magnetic sensing probe for levofloxacin determination integrates porous graphene, selective polymer and graphene quantum dots

• Published in: Journal of pharmaceutical and biomedical analysis 2021-10, Vol.205, p.114316-114316, Article 114316
• Main Authors: Chansud, Nutnicha, Longnapa, Noppanut, Bunkoed, Opas
• Format: Article
• Language: English
• Subjects: Graphene quantum dots; Levofloxacin; MIP; Nanoprobe; Optosensor; Porous graphene
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2021.114316

[Display omitted] •A nanohybrid fluorescent probe was designed and fabricated to detect levofloxacin.•The nanohybrid probe integrated porous graphene, Fe3O4@SiO2 and GQDs into MIP.•The probe showed good sensitivity, selectivity, binding and analyte enrichment.•The probe detected levofloxacin in milk with recoveries between 91.8 % and 100.5 %. A nanohybrid magnetic fluorescent sensing probe was designed and fabricated for ultrasensitive and selective determination of levofloxacin. The probe integrated porous graphene (PGr), magnetite (Fe3O4) nanoparticles and graphene quantum dots (GQDs) into selective molecularly imprinted polymer (MIP). The developed probe was sensitive, selective, and its binding ability enriched levofloxacin in complex samples. The fabrication strategy was evaluated to achieve the best performance and the synthesized sensing probe was characterized. In the best condition, the fluorescence emission of the probe was quenched linearly from 0.10 to 25.0 μg L-1 of levofloxacin and the limit of detection was 0.03 μg L-1. The quenching of fluorescence was not affected by the analog compounds ciprofloxacin, lomefloxacin, marbofloxacin and sarafloxacin. The imprinting factor of the developed nanohybrid sensing probe was 4.26. The developed probe was utilized to detect levofloxacin in milk and recoveries between 91.8 % and 100.5 % were achieved with RSDs