Combining Bone Collagen Matrix with hUC-MSCs for Application to Alveolar Process Cleft in a Rabbit Model

Background Most materials used clinically for filling severe bone defects either cannot induce bone re-generation or exhibit low bone conversion, therefore, their therapeutic effects are limited. Human umbilical cord mesenchymal stem cells (hUC-MSCs) exhibit good osteoinduction. However, the mechani...

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Bibliographic Details
Published in:Stem cell reviews and reports 2023, Vol.19 (1), p.133-154
Main Authors: Sun, Xue-Cheng, Wang, Hu, Zhang, Dan, Li, Jian-Hui, Yin, Li-Qiang, Yan, Yu-Fang, Ma, Xu, Xia, Hong-Fei
Format: Article
Language:English
Subjects:
Alveolar bone
Alveolar Process
Animals
Apoptosis
Biomedical and Life Sciences
Biomedical Engineering and Bioengineering
Bone growth
Bone matrix
Bone morphogenetic protein 2
Bone Regeneration
Bone surgery
Cell Biology
Chondrocytes
Collagen
Collagen (type I)
Computed tomography
Humans
Life Sciences
Mesenchymal Stem Cells
Mesenchyme
Osteogenesis
Rabbits
Regeneration
Regenerative Medicine/Tissue Engineering
Stem Cells
Surgery
Umbilical cord
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Summary:Background Most materials used clinically for filling severe bone defects either cannot induce bone re-generation or exhibit low bone conversion, therefore, their therapeutic effects are limited. Human umbilical cord mesenchymal stem cells (hUC-MSCs) exhibit good osteoinduction. However, the mechanism by which combining a heterogeneous bone collagen matrix with hUC-MSCs to repair the bone defects of alveolar process clefts remains unclear. Methods A rabbit alveolar process cleft model was established by removing the bone tissue from the left maxillary bone. Forty-eight young Japanese white rabbits (JWRs) were divided into normal, control, material and MSCs groups. An equal volume of a bone collagen matrix alone or combined with hUC-MSCs was implanted in the defect. X-ray, micro-focus computerized tomography (micro-CT), blood analysis, histochemical staining and TUNEL were used to detect the newly formed bone in the defect area at 3 and 6 months after the surgery. Results The bone formation rate obtained from the skull tissue in MSCs group was significantly higher than that in control group at 3 months ( P  
ISSN:2629-3269
2629-3277
DOI:10.1007/s12015-021-10221-y