Persistence of Semen on five different fabric types in various water environments

• Published in: Forensic science international 2021-10, Vol.327, p.110944-110944, Article 110944
• Main Authors: Nabi, Ahmed Ghulam, Mateen, Rana Muhammad, Khalid, Aqsa, Tariq, Asma, Parveen, Rukhsana
• Format: Article
• Language: English
• Subjects: Acid phosphatase; Acids; Cotton; Crime; Crime scene; Drinking water; Enzymes; Fluorescence; Forensic science; Forensic sciences; Forensics; Immersion; Indigo; Light sources; Linen; Persistence; Phosphatase; Polyesters; Rape; Recreation; Rivers; Semen; Seminal fluid; Serology; Sex crimes; Sexual assault; Silk; Sperm; Spermatozoa; Stains & staining; Submerging; Swimming pools; Victims of crime
• ISSN: 0379-0738; 1872-6283
• DOI: 10.1016/j.forsciint.2021.110944

Evidence collected from biological fluids obtained from a crime scene is essentially important in forensic cases. A potential profile can be generated from these obtained samples and this can help in identifying the victims and/or suspects of sexual assault. The water environments selected for this study are all related to the potential crime scenes from which there is a possibility of finding a dead body or clothing of a sexual assault victim. Tap water, River water, Swimming pool water, and Canal water were selected. Fabric types selected were khaddar, linen, silk, polyester, and chiffon. Detection of seminal stains was carried out by three methods; Alternate Light Source (ALS), Acid phosphatase (AP) testing, and Kernechtrot-Picro-indigo-carmine (KPIC) testing. These tests were performed for each fabric type in each water environment after regular intervals, 24 h, 48 h, 72 h, 4 days, 7 days, and 14 days. This study aimed to compare the ability of five types of fabrics to retain seminal material after immersion in four different types of water environments. Fluorescence was only detected in tap water-soaked silk fabric after 14 days of immersion. Seminal fluid was detected in khaddar, chiffon, silk, and polyester in samples immersed for 14 days in tap water. Spermatozoa were retained by khaddar and silk immersed in tap water, Polyester fabric in tap and river water, Chiffon in only river water and Linen in swimming pool water when immersed for 14 days. However, fluorescence, seminal fluid or spermatozoa were not detected in linen fabric regardless of all the afore mentioned variables. •Evidence collected from biological fluids obtained from a crime scene is essentially important in forensic investigations.•Five types of fabrics to retain seminal material after immersion in four different types of water environments were evaluated.•Presumptive and confirmatory tests indicated that semen retention is dependent on fabric type.