Coenzyme Q10 ameliorates the quality of mouse oocytes during in vitro culture

Oxidative stress causes several diseases and dysfunctions in cells, including oocytes. Clearly, oxidative stress influences oocyte quality during in vitro maturation and fertilization. Here we tested the ability of coenzyme Q10 (CoQ10) to reduce reactive oxygen species (ROS) and improve mouse oocyte...

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Bibliographic Details
Published in:Zygote (Cambridge) 2022-04, Vol.30 (2), p.249-257
Main Authors: Lee, Chan Hee, Kang, Min Kook, Sohn, Dong Hyun, Kim, Hye Min, Yang, Juri, Han, Seung Jin
Format: Article
Language:English
Subjects:
Aging
Alzheimer's disease
Animals
Cell culture
Cell cycle
Coenzyme Q10
Cumulus Cells
Cytochrome
Embryos
Enzymes
Extrusion
Female
Fertilization
Fertilization in Vitro - methods
Gametocytes
Gene expression
In vitro fertilization
In Vitro Oocyte Maturation Techniques - methods
Infertility
Maturation
Mice
Oocytes
Ovaries
Ovulation
Oxidation
Oxidative stress
Parthenogenesis
Phosphorylation
Reactive oxygen species
SIRT1 protein
Statistical analysis
Ubiquinone - analogs & derivatives
Zona pellucida
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Summary:Oxidative stress causes several diseases and dysfunctions in cells, including oocytes. Clearly, oxidative stress influences oocyte quality during in vitro maturation and fertilization. Here we tested the ability of coenzyme Q10 (CoQ10) to reduce reactive oxygen species (ROS) and improve mouse oocyte quality during in vitro culture. Treatment with 50 μM CoQ10 efficiently reduced ROS levels in oocytes cultured in vitro. The fertilizable form of an oocyte usually contains a cortical granule-free domain (CGFD). CoQ10 enhanced the ratio of CGFD-oocytes from 35% to 45%. However, the hardening of the zona pellucida in oocytes was not affected by CoQ10 treatment. The in vitro maturation capacity of oocytes, which was determined by the first polar body extrusion, was enhanced from 48.9% to 75.7% by the addition of CoQ10 to the culture medium. During the parthenogenesis process, the number of two-cell embryos was increased by CoQ10 from 43.5% to 67.3%. Additionally, treatment with CoQ10 increased the expression of Bcl2 and Sirt1 in cumulus cells. These results suggested that CoQ10 had a positive effect on ROS reduction, maturation rate and two-cell embryo formation in mouse oocyte culture.
ISSN:0967-1994
1469-8730
DOI:10.1017/S0967199421000617