Covalent Organic Framework-Derived Carbonous Nanoprobes for Cancer Cell Imaging

Covalent organic frameworks (COFs) have emerged as promising materials for biomedical applications, but their functions remain to be explored and the potential toxicity concerns should be resolved. Herein, it is presented that carbonization significantly enhances the fluorescence quenching efficienc...

Full description

Saved in:
Bibliographic Details
Published in:ACS applied materials & interfaces 2021-09, Vol.13 (35), p.41498-41506
Main Authors: Gao, Peng, Shen, Xiaoying, Liu, Xiaohan, Cui, Bingjie, Wang, Mengzhen, Wan, Xiuyan, Li, Na, Tang, Bo
Format: Article
Language:English
Subjects:
Biological and Medical Applications of Materials and Interfaces
Biomarkers, Tumor - analysis
Biomarkers, Tumor - genetics
Carbon - chemistry
Carbon - toxicity
Cell Line, Tumor
DNA - chemistry
DNA - toxicity
Fluorescent Dyes - chemistry
Fluorescent Dyes - toxicity
Humans
Immobilized Nucleic Acids - chemistry
Immobilized Nucleic Acids - toxicity
Metal-Organic Frameworks - chemistry
Metal-Organic Frameworks - toxicity
Microscopy, Confocal
Microscopy, Fluorescence
Nanoparticles - chemistry
Nanoparticles - toxicity
Neoplasms - diagnostic imaging
RNA, Messenger - analysis
RNA, Messenger - genetics
Survivin - genetics
Thymidine Kinase - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Covalent organic frameworks (COFs) have emerged as promising materials for biomedical applications, but their functions remain to be explored and the potential toxicity concerns should be resolved. Herein, it is presented that carbonization significantly enhances the fluorescence quenching efficiency and aqueous stability of nanoscale COFs. The probes prepared by physisorbing dye-labeled nucleic acid recognition sequences onto the carbonized COF nanoparticles (termed C-COF) were employed for cell imaging, which could effectively light up biomarkers (survivin and TK1 mRNA) in living cells. The C-COF has enhanced photothermal conversion capacity, indicating that the probes are also promising candidates for photothermal therapy. The potential toxicity concern from the aromatic rigid building units of COFs was detoured by carbonization. Overall, carbonization is a promising strategy for developing biocompatible and multifunctional COF-derived nanoprobes for biomedical applications. This work may inspire more versatile COF-derived nanoprobes for bioanalysis and nanomedicine.
ISSN:1944-8244
1944-8252
DOI:10.1021/acsami.1c14998