Antibiotic resistance of culturable heterotrophic bacteria isolated from shrimp (Penaeus vannamei) aquaculture ponds

• Published in: Marine pollution bulletin 2021-11, Vol.172, p.112887-112887, Article 112887
• Main Authors: Nadella, Ranjit Kumar, Panda, Satyen Kumar, Madhusudana Rao, B., Pani Prasad, K., Raman, R.P., Mothadaka, Mukteswar Prasad
• Format: Article
• Language: English
• Subjects: Antibiotic resistance; Antibiotics; Antibiotics usage; Antimicrobial resistance; Antimicrobial resistance (AMR); Aquaculture; Aquaculture environment; Bacteria; Chloramphenicol; Chloromycetin; Ciprofloxacin; Cotrimoxazole; Disease resistance; Drug resistance; Erythromycin; Gram-negative bacteria; Heterotrophic bacteria; Litopenaeus vannamei; Marine crustaceans; Morphotintorial groups; Multidrug resistance; Oxytetracycline; Ponds; Sediment; Sediment samples; Shrimp culture; Shrimp farming; Water quality parameters
• ISSN: 0025-326X; 1879-3363
• DOI: 10.1016/j.marpolbul.2021.112887

Shrimp aquaculture is one of the fastest growing food-producing avenues, where antibiotics usage has become an issue of great concern due to the development of antimicrobial resistance in bacteria. A total of 2304 bacterial isolates from 192 samples (sediment, water, shrimp, and source water) from Andhra Pradesh, India were screened. Antibiotic resistance of bacterial isolates was highest for oxytetracycline (23.4%) followed by erythromycin (12.7%), co-trimoxazole (10%) ciprofloxacin (9.6%), and chloramphenicol (6%), of which 11.9% isolates were multi-drug resistant. Bacterial isolates from shrimp (26.7%), water (23.9%), and sediment (19.6%) samples exhibited more resistance (p ≤ 0.05) towards oxytetracycline. Higher antibacterial resistance was observed from samples of southern Andhra Pradesh (locations L6 and L7). Gram negative bacteria were more prevalent (64%) and showed significantly (p ≤ 0.01) higher resistance. This study indicated the wider distribution of antibiotic-resistant bacteria in shrimp aquaculture ponds with potential risk to humans and the environment. •Antibiotic-resistant bacteria were widely distributed in shrimp aquaculture ponds.•Resistance towards oxytetracycline (23.4%) was relatively higher.•Multi-drug resistance was observed in 11.85% of the resistant bacterial isolates.•Two out of 12 locations L6 and L7 harbored relatively more drug-resistant bacteria.•Gram negative bacteria exhibited higher resistance in all the locations of study.