Knockdown of lncRNA SNHG15 Ameliorates Oxygen and Glucose Deprivation (OGD)-Induced Neuronal Injury via Regulating the miR-9-5p/TIPARP Axis

• Published in: Biochemical genetics 2022-04, Vol.60 (2), p.755-769
• Main Authors: Shen, Bin, Wang, Lan, Xu, Yuejun, Wang, Hongwei, He, Shiyi
• Format: Article
• Language: English
• Subjects: Adenosine diphosphate; Animals; Apoptosis; Apoptosis - genetics; Assaying; Biochemistry; Biomedical and Life Sciences; Biomedicine; Biotin; Cell injury; Cell viability; Cerebral blood flow; Cerebrovascular diseases; Deprivation; Flow cytometry; Glucose; Glucose - metabolism; Human Genetics; Injuries; Ischemia; Medical Microbiology; Mice; MicroRNAs - genetics; MicroRNAs - metabolism; Neurons - metabolism; Neurons - pathology; Non-coding RNA; Nucleoli; Occlusion; Original Article; Oxygen; Oxygen - metabolism; Poly(ADP-ribose) Polymerases - genetics; Polymerase chain reaction; Ribonucleic acid; Ribose; RNA; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; snoRNA; Stroke; Zoology
• ISSN: 0006-2928; 1573-4927
• DOI: 10.1007/s10528-021-10121-3

Stroke is a cerebrovascular disease with impaired nerve function. Long non-coding RNA (lncRNA) is considered to be an important regulator of various diseases. Nevertheless, the role of lncRNA small nucleolar RNA host gene 15 (SNHG15) in cerebral ischemia injury induced by stroke is still unclear. Cell-counting kit 8 assay and flow cytometry were used to detect cell viability and apoptosis, respectively. The caspase3 activity of cells was measured using Caspase3 Activity Assay Kit. Besides, the protein levels of apoptosis markers and TCCD-induced poly (ADP)-ribose polymerase (TIPARP) were determined using western blot analysis. Moreover, quantitative real-time polymerase chain reaction was employed to examine the relative expression of SNHG15 and miR-9-5p. Furthermore, dual-luciferase reporter assay was used to assess the interaction between miR-9-5p and SNHG15 or TIPARP. In addition, biotin-labeled RNA pull-down assay was performed to evaluate the interaction between miR-9-5p and SNHG15 further. Middle cerebral artery occlusion (MCAO) model was constructed to further explore the role of SNHG15 in neuronal injury in vivo. Our data showed that oxygen and glucose deprivation (OGD) could induce N-2a cell injury and enhance SNHG15 expression. Silenced SNHG15 could promote the viability and suppress the apoptosis of OGD-induced N-2a cells. Also, SNHG15 knockdown also could alleviate the neuronal injury of MCAO mice. Mechanistically, SNHG15 could sponge miR-9-5p, and miR-9-5p could target TIPARP. Further experiments revealed that miR-9-5p inhibition or TIPARP overexpression could reverse the suppressive effect of SNHG15 knockdown on OGD-induced N-2a cell injury. Our findings indicated that SNHG15 knockdown inhibited neuronal injury through the miR-9-5p/TIPARP axis, suggesting that SNHG15 might be a potential target for cerebral ischemia injury induced by stroke.