A novel surgical toxicological-free model of diaphragmatic hernia in fetal rats

Background Teratogen-induced congenital diaphragmatic hernia (CDH) rat models are commonly used to study the pathophysiology. We have created a new and reliable surgically induced diaphragmatic hernia (DH) model to obtain a purely mechanical DH rat model, and avoid the confounding teratogen-induced...

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Bibliographic Details
Published in:Pediatric research 2022-07, Vol.92 (1), p.118-124
Main Authors: Sbragia, Lourenço, Oria, Marc, Scorletti, Federico, Romero Lopez, Maria del Mar, Schmidt, Augusto F., Levy, Brittany, Peiro, Jose L.
Format: Article
Language:English
Subjects:
Abdomen
Basic Science Article
Diaphragm (Anatomy)
Fetuses
Hernias
Hospitals
Laboratory animals
Lungs
Medicine
Medicine & Public Health
Pathophysiology
Pediatric Surgery
Pediatrics
Rodents
Thoracic surgery
Thorax
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Summary:Background Teratogen-induced congenital diaphragmatic hernia (CDH) rat models are commonly used to study the pathophysiology. We have created a new and reliable surgically induced diaphragmatic hernia (DH) model to obtain a purely mechanical DH rat model, and avoid the confounding teratogen-induced effects on the lung development. Methods Fetal DH was surgically created on fetuses at E18.5 and harvested at E21.5 in rats. Four groups were evaluated ( n  = 16): control (CONT), control exposed to Nitrofen (CONT NIT), DH surgically created (DH SURG), and CDH Nitrofen (CDH NIT). Body weight, total lung weights, and their ratio (BW, TLW, and TLBR) were compared. Air space (AS), parenchyma (PA), total protein, and DNA contents were measured to verify lung hypoplasia. Medial wall thickness (MWT) of pulmonary arterioles was also analyzed. Results DH SURG showed significant hypoplasia (decreased in total protein and DNA) vs CONT ( p  
ISSN:0031-3998
1530-0447
DOI:10.1038/s41390-021-01702-4