circPTPN22 attenuates immune microenvironment of pancreatic cancer via STAT3 acetylation

Accumulating research implicated that circular RNAs exhibited significant roles in cancer development. Nonetheless, the role regarding circPTPN22 in pancreatic cancer remains unclear. Expression of circPTPN22 in pancreatic cancer cell lines and normal cells was determined with quantitative real-time...

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Bibliographic Details
Published in:Cancer gene therapy 2023-04, Vol.30 (4), p.559-566
Main Authors: He, Yuan, Han, Pengyong, Chen, Chuang, Xie, Shuzhe, Zhang, Huiqing, Song, Yingming, Hu, Hao, Zhao, Qiang, Lian, Changhong
Format: Article
Language:English
Subjects:
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Acetylation
Antibodies
Apoptosis
Biomedical and Life Sciences
Biomedicine
Cancer therapies
Cell Line, Tumor
Cell proliferation
Cell Proliferation - genetics
Cells
Chemotherapy
Flow cytometry
Gene Expression
Gene Therapy
Humans
Immunoprecipitation
Immunotherapy
Medical prognosis
Metastasis
Microenvironments
Pancreatic cancer
Pancreatic Neoplasms
Pancreatic Neoplasms - genetics
Pancreatic Neoplasms - metabolism
Proteins
Radiation therapy
RNA
SIRT1 protein
Sirtuin 1 - genetics
Sirtuin 1 - metabolism
Stat3 protein
STAT3 Transcription Factor - genetics
STAT3 Transcription Factor - metabolism
Surgery
Tumor cell lines
Tumor Microenvironment - genetics
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Summary:Accumulating research implicated that circular RNAs exhibited significant roles in cancer development. Nonetheless, the role regarding circPTPN22 in pancreatic cancer remains unclear. Expression of circPTPN22 in pancreatic cancer cell lines and normal cells was determined with quantitative real-time PCR (qRT-PCR). Cell counting kit-8 assay and colony formation assay were used to measure the proliferation of pancreatic cancer cells. RNA immunoprecipitation and Western blot were employed for investigation the binding between circPTPN22 and STAT3. circPTPN22 expression was highly upregulated in pancreatic cancer tissues and cell lines. Knockdown of circPTPN22 inhibited cell proliferation and attenuates pancreatic cancer immune microenvironment. Furthermore, STAT3 acetylation was involved in these effects. circPTPN22 promoted STAT3 acetylation via inhibiting STAT3/SIRT1 interaction. circPTPN22 attenuates pancreatic cancer immune microenvironment by promoting STAT3 acetylation via inhibiting STAT3/SIRT1 interaction.
ISSN:0929-1903
1476-5500
DOI:10.1038/s41417-021-00382-w