Electrochemical immunoplatform to assist in the diagnosis of oral cancer through the determination of CYFRA 21.1 biomarker in human saliva samples: Preparation of a novel portable biosensor toward non‐invasive diagnosis of oral cancer

In this study, a novel, low‐cost, and flexible paper‐based electrochemical immunosensor was developed for the bioanalysis of Cyfra 21.1 biomarker in human saliva samples by using stabilization of synthesis Ag nano‐ink on the surface of paper using pen‐on‐paper technology. The employed electrochemica...

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Bibliographic Details
Published in:Journal of molecular recognition 2021-12, Vol.34 (12), p.e2932-n/a
Main Authors: Tofighi, Fahimeh Bageri, Saadati, Arezoo, Kholafazad‐kordasht, Houman, Farshchi, Fatemeh, Hasanzadeh, Mohammad, Samiei, Mohammad
Format: Article
Language:English
Subjects:
advanced nanomaterial
Antigens, Neoplasm
Biomarkers
biomedical analysis
Biosensing Techniques - methods
Biosensors
biotechnology
Cancer
Cyfra 21.1 biomarker
Diagnosis
Electrochemical Techniques - methods
Electrochemistry
Humans
Immunoassay - methods
immunosensor
Immunosensors
Keratin-19
Limit of Detection
Mouth Neoplasms - diagnosis
Oral cancer
Portability
protein
Saliva
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Summary:In this study, a novel, low‐cost, and flexible paper‐based electrochemical immunosensor was developed for the bioanalysis of Cyfra 21.1 biomarker in human saliva samples by using stabilization of synthesis Ag nano‐ink on the surface of paper using pen‐on‐paper technology. The employed electrochemical techniques for the evaluation of immunoplatform performance were differential pulse voltammetry and chronoamperometry. Also, the prepared immunosensor showed great ability in the determination of Cyfra21.1 in human saliva specimens. Under the optimized conditions, the obtained linear range was from 0.0025 to 10 ng/mL, and the obtained LLOQ was 0.0025 ng/mL. The developed immunosensor is easy to prepare, sensitive, cost‐effective, portable, and simple. So proposed immunoplatform can be an accomplished biodevice in clinical laboratories. The proposed paper‐based immunosensor could be a hopefully new and cheap tool for the diagnosis of other biomarkers. Also, the prepared immunosensor showed great ability in the determination of Cyfra21.1 biomarker in human saliva specimens.
ISSN:0952-3499
1099-1352
DOI:10.1002/jmr.2932