ExoSD chips for high-purity immunomagnetic separation and high-sensitivity detection of gastric cancer cell-derived exosomes

• Published in: Biosensors & bioelectronics 2021-12, Vol.194, p.113594-113594, Article 113594
• Main Authors: Yu, Zixian, Lin, Shujing, Xia, Fangfang, Liu, Yanlei, Zhang, Di, Wang, Fei, Wang, Yanpu, Li, Qichao, Niu, Jiaqi, Cao, Chengxi, Cui, Daxiang, Sheng, Nengquan, Ren, Jiazi, Wang, Zhigang, Chen, Di
• Format: Article
• Language: English
• Subjects: Exosome detection; Exosome separation; Exosomes; Microfluidic chip; Microvesicles
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2021.113594

Gastric cancer cell-derived exosomes as biomarkers have a very high application potential to the non-invasive detection of early-stage gastric cancer. However, the small size of exosomes (30–150 nm) results in huge challenges in separating and detecting them from complex media (e.g., plasma, urine, saliva, and cell culture supernatant). Here we proposed a highly integrated exosome separation and detection (ExoSD) chip to immunomagnetic separate exosomes from cell culture supernatant in a manner of continuous flow, and to immunofluorescence detect gastric cancer cell-derived exosomes with high sensitivity. The ExoSD chip has achieved a high exosome recovery (>80%) and purity (>83%) at the injection rate of 4.8 mL/h. Furthermore, experimental results based on clinical serum samples of patients with gastric cancer (stages I and II) show that the detection rate of the ExoSD chip is as high as 70%. The proposed ExoSD chip has been successfully demonstrated as a cutting-edge platform for exosomes separation and detection. It can be served as a versatile platform to extend to the applications of separation and detection of the other cell-derived exosomes or cells. •A Ni comb-like structure is designed to construct the strong gradient magnetic field to ensure the exosome recovery rate.•The microfilter can prevent the impurity from one stream diffuse into the others and ensure exosome separation purity.•Ni material is used to fabricate a micro cylinder array to capturing Exosomes@IMNPs with high efficiency for rapid detection.