1,5-anhydroglucitol biosensor based on light-addressable potentiometric sensor with RGO-CS-Fc/Au NPs nanohybrids

• Published in: Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2021-12, Vol.142, p.107938-107938, Article 107938
• Main Authors: Liang, Jintao, Shi, Xiaohang, Feng, Huafu, Chen, Min, Li, Wenzhan, Lai, Junxiang, Hu, Weipeng, Li, Guiyin
• Format: Article
• Language: English
• Subjects: 1,5-anhydroglucitol; Biosensing Techniques - methods; Biosensors; Catalytic oxidation; Chitosan; Correlation coefficient; Correlation coefficients; Deoxyglucose - blood; Electrochemical Techniques - methods; Gold - chemistry; Gold nanoparticles; Graphene; Humans; Hydrogen peroxide; Ions; Iron; Light-addressable potentiometric sensor; Membranes; Metal Nanoparticles - chemistry; Oxidation; Pyranose oxidase; Reduced graphene oxide-chitosan-ferrocene nanohybrids
• ISSN: 1567-5394; 1878-562X
• DOI: 10.1016/j.bioelechem.2021.107938

[Display omitted] •1,5-AG biosensor was prepared based on LAPS with RGO-CS-Fc/Au NPs nanohybrids.•1,5-AG biosensor was characterized by SEM and XPS.•1,5-AG biosensor demonstrated linearity from 10 µg·mL−1 to 350 µg·mL−1 with LOD of 10 µg·mL−1.•1,5-AG biosensor had good selectivity, stability, reproducibility and recoveries. In this paper, a novel silicon-based light-addressable potentiometric sensor (LAPS) has been designed for the detection of 1,5-anhydroglucitol (1,5-AG) in human serum. Reduced graphene oxide-chitosan-ferrocene (RGO-CS-Fc)/AuNPs nanohybrids and pyranose oxidase (PROD) enzyme is used to fabricate biological sensitive membrane unit by layer-by-layer assembly technology. When a bias voltage is provided to the LAPS system, the catalytic oxidation reaction between 1,5-AG and PROD to produce H2O2. The by-product H2O2 can oxidize Fc(Fe2+) ions in RGO-CS-Fc nanohybrids into Fc(Fe3+) ions, which cause the potential of the sensitive membrane surface to change and the potential shift of I-V curve will generate a corresponding offset response. Under the optimal conditions, the potential shift of the LAPS is linearly related to the concentration of 1,5-AG at 10 µg·mL−1 –350 µg·mL−1 with the correlation coefficient of 0.97414. The sensitivity is 0.44273 mV/µg·mL−1 and the lowest detection limit is 10 µg·mL−1. In addition, the biosensor showed good specificity, acceptable stability and satisfactory recovery rates (91.28%-107.66%), which would be a potential testing methods in actual clinical samples.