Menstrual blood CD146+ mesenchymal stem cells reduced fibrosis rate in the rat model of premature ovarian failure

Here, the regenerative potential of menstrual blood–derived mesenchymal stem cells (MenSCs) was examined on restoration of premature ovarian failure (POF) ovaries in rats' POF model. Freshly isolated CD146+ MenSCs using magnetic‐activated cell storing method were immediately injected into ovari...

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Bibliographic Details
Published in:Cell biochemistry and function 2021-12, Vol.39 (8), p.998-1008
Main Authors: Yamchi, Nahideh Nazdikbin, Rahbarghazi, Reza, Bedate, Alberto Miranda, Mahdipour, Mahdi, Nouri, Mohammad, Khanbabaee, Ramazan
Format: Article
Language:English
Subjects:
Animals
Blood
Blood levels
CD146 Antigen - blood
CD146 Antigen - metabolism
Ceftriaxone
Cell lineage
Disease Models, Animal
Female
Fertility
Fibrosis
Fibrosis - metabolism
Fibrosis - pathology
Follicle-stimulating hormone
Follicles
Gene expression
Gene regulation
menstrual‐derived stem cells
Menstruation
Mesenchymal stem cells
Mesenchymal Stem Cells - cytology
ovarian rejuvenation
Ovaries
premature ovarian failure
Primary Ovarian Insufficiency - metabolism
Primary Ovarian Insufficiency - pathology
Rats
Rats, Wistar
Reduction
Reproductive status
Smad protein
Stem cell transplantation
Stem cells
Transcription
Transplantation
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Summary:Here, the regenerative potential of menstrual blood–derived mesenchymal stem cells (MenSCs) was examined on restoration of premature ovarian failure (POF) ovaries in rats' POF model. Freshly isolated CD146+ MenSCs using magnetic‐activated cell storing method were immediately injected into ovaries of POF rats. Four and eight weeks after cell administration, both ovarian tissues were sampled for histological examination and the expression of fibrosis‐related genes. Serum samples were also prepared for hormonal analysis. At the endpoint, mating trials were performed to assess the fertility of POF rats following MenSC transplantation. Histopathological examination revealed the induction of POF after Ceftriaxone injection by increasing atretic follicles and abnormal morphologies. MenSCs transplantation increased the number of normal follicles and coincided with the reduction of follicular atresia. Biochemical analyses exhibited the reduction and increase of systemic follicle‐stimulating hormone (FSH) and E2 respectively after MenSCs transplantation compared to the POF rats (P 
ISSN:0263-6484
1099-0844
DOI:10.1002/cbf.3669