Bio-hybrid hydrogel comprising collagen-capped silver nanoparticles and melatonin for accelerated tissue regeneration in skin defects

• Published in: Materials Science & Engineering C 2021-09, Vol.128, p.112328-112328, Article 112328
• Main Authors: Ragothaman, Murali, Kannan Villalan, Arivizhivendhan, Dhanasekaran, Anuradha, Palanisamy, Thanikaivelan
• Format: Article
• Language: English
• Subjects: Aminated xanthan gum; Angiogenesis; Animal tissues; Anti-inflammatory agents; Antiinfectives and antibacterials; Antimicrobial; Antioxidants; Biocompatibility; Chronic infection; Collagen; Defects; Drug delivery; Drug delivery systems; Ethylenediamine; Hybrid systems; Hydrogels; In vivo methods and tests; Inflammation; Materials science; Melatonin; Morphology; Multidrug resistance; Nanoparticles; Regeneration; Rheological properties; Rheology; Silver; Silver nanoparticles; Stabilizers (agents); Synergistic effect; Synthesis; System effectiveness; Tissue engineering; Tissue regeneration; Wounds; Xanthan; Xanthan gum
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2021.112328

Hydrogel-based drug delivery systems have emerged as a promising platform for chronic tissue defects owing to their inherent ability to inhibit pathogenic infection and accelerate rapid tissue regeneration. Here, we fabricated a stable bio-hybrid hydrogel system comprising collagen, aminated xanthan gum, bio-capped silver nanoparticles and melatonin with antimicrobial, antioxidant and anti-inflammatory properties. Highly colloidal bio-capped silver nanoparticles were synthesized using collagen as a reducing cum stabilizing agent for the first time while aminated xanthan gum was synthesized using ethylenediamine treatment on xanthan gum. The synthesized bio-hybrid hydrogel exhibits better gelation, surface morphology, rheology and degelation properties. In vitro assessment of bio-hybrid hydrogel demonstrates excellent bactericidal efficiency against both common wound and multidrug-resistant pathogens and biocompatibility properties. In vivo animal studies demonstrate rapid tissue regeneration, collagen deposition and angiogenesis at the wound site predominantly due to the synergistic effect of silver nanoparticles and melatonin in the hydrogel. This study paves the way for developing biologically functional bio-nano hydrogel systems for promoting effective care for various ailments, including infected chronic wounds. [Display omitted] •Green synthesis of AGNP was derived from collagen biomolecules for the first time.•Aminated xanthan gum was synthesized by treating ethylenediamine in xanthan gum.•Bio-hybrid hydrogel was prepared with a synergistic AGNP and melatonin combination.•Hydrogels displayed excellent antimicrobial and biocompatibility property.•In vivo animal studies of hydrogel exhibited rapid wound closure and regeneration.