Melittin suppresses epithelial-mesenchymal transition and metastasis in human gastric cancer AGS cells via regulating Wnt/BMP associated pathway

Gastric cancer has a poor prognosis; once cancer has metastasized, it can easily lead to patient death. Melittin is one of the major components extracted from the bee venom. It has been shown that melittin emerges antitumor activities against many human cancer cell lines. Our results indicated that...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2021-11, Vol.85 (11), p.2250-2262
Main Authors: Huang, Jye-Yu, Peng, Shu-Fen, Chueh, Fu-Shin, Chen, Po-Yuan, Huang, Yi-Ping, Huang, Wen-Wen, Chung, Jing-Gung
Format: Article
Language:English
Subjects:
Bone Morphogenetic Proteins - metabolism
Cell Adhesion - drug effects
Cell Line, Tumor
Cell Movement - drug effects
Cell Survival - drug effects
Epithelial-Mesenchymal Transition - drug effects
Humans
Matrix Metalloproteinase 2 - genetics
Matrix Metalloproteinase 2 - metabolism
Matrix Metalloproteinase 9 - genetics
Matrix Metalloproteinase 9 - metabolism
Melitten - pharmacology
Neoplasm Invasiveness
Neoplasm Metastasis
Stomach Neoplasms - drug therapy
Stomach Neoplasms - metabolism
Stomach Neoplasms - pathology
Wnt Signaling Pathway - drug effects
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Summary:Gastric cancer has a poor prognosis; once cancer has metastasized, it can easily lead to patient death. Melittin is one of the major components extracted from the bee venom. It has been shown that melittin emerges antitumor activities against many human cancer cell lines. Our results indicated that melittin at 0.2-0.5 µm significantly reduced total cell viability in human gastric cancer AGS cells. At low concentrations (0.05-0.15 µm), melittin displayed antimetastasis effects and inhibited cell adhesion and colony formation. Besides, it inhibited cell motility and suppressed cell migration and invasion. Melittin inhibited the activities of MMP-2 and MMP-9 and the integrity of cell membrane in AGS cells. Furthermore, Western blotting results showed that melittin decreased the protein expressions of Wnt/BMP and MMP-2 signaling pathways. Based on these observations, melittin inhibited cell migration and invasion of AGS cells through multiple signaling pathways. It may be used to treat metastasized gastric cancers in the future.
ISSN:1347-6947
1347-6947
DOI:10.1093/bbb/zbab153