16S rDNA PCR for the aetiological diagnosis of culture-negative infective endocarditis

Introduction Culture-negative infective endocarditis (IE) accounts for 7–31% of all cases. Metagenomics has contributed to improving the aetiological diagnosis of IE patients undergoing valve surgery. We assessed the impact of 16S ribosomal DNA gene polymerase chain reaction (16S rDNA PCR) in the ae...

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Bibliographic Details
Published in:Infection 2022-02, Vol.50 (1), p.243-249
Main Authors: Anton-Vazquez, Vanesa, Dworakowski, Rafal, Cannata, Antonio, Amin-Youssef, George, Gunning, Margaret, Papachristidis, Alexandros, MacCarthy, Phil, Baghai, Max, Deshpande, Ranjit, Khan, Habib, Byrne, Jonathan, Fife, Amanda
Format: Article
Language:English
Subjects:
Aorta
Aortic valve
Bacteria
Diagnosis
DNA, Ribosomal - genetics
Endocarditis
Endocarditis - diagnosis
Endocarditis, Bacterial - diagnosis
Etiology
Family Medicine
General Practice
Heart valves
Humans
Infectious Diseases
Internal Medicine
Male
Medical diagnosis
Medicine
Medicine & Public Health
Metagenomics
Middle Aged
Nucleic acids
Original Paper
Patients
Polymerase Chain Reaction
Retrospective Studies
Ribosomal DNA
RNA, Ribosomal, 16S - genetics
rRNA 16S
Tropheryma whipplei
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Summary:Introduction Culture-negative infective endocarditis (IE) accounts for 7–31% of all cases. Metagenomics has contributed to improving the aetiological diagnosis of IE patients undergoing valve surgery. We assessed the impact of 16S ribosomal DNA gene polymerase chain reaction (16S rDNA PCR) in the aetiological diagnosis of culture-negative IE. Methods Between January 2016 and January 2020, clinical data from culture-negative IE patients were reviewed retrospectively. Identification of bacteria was performed using 16S rDNA PCR in heart valve specimens. Results 36 out of 313 patients (12%) with culture-negative IE had their valve tissue specimens submitted for 16S rDNA PCR. 16S rDNA PCR detected and identified bacterial nucleic acid in heart valve tissue significantly more frequently compared to valve culture alone 25(70%) vs 5(12%); p  
ISSN:0300-8126
1439-0973
DOI:10.1007/s15010-021-01690-x