Amphioxus ribosomal proteins RPS15, RPS18, RPS19 and RPS30-precursor act as immune effectors via killing or agglutinating bacteria

Previous studies show that some ribosomal proteins perform immune effector functions via killing bacteria directly. However, it remains largely unknown about other effector functions of ribosomal proteins during a bacterial infection. In this study, we expressed and purified four ribosomal proteins...

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Bibliographic Details
Published in:Fish & shellfish immunology 2021-11, Vol.118, p.147-154
Main Authors: Chen, Chaoyi, Yuan, Jianrui, Ji, Guangdong, Zhang, Shicui, Gao, Zhan
Format: Article
Language:English
Subjects:
Agglutination
Amphioxus
Animals
Bacteria
Bactericidal protein
Escherichia coli
Homicide
Lancelets - genetics
Ribosomal protein
Ribosomal Proteins - genetics
Staphylococcus aureus
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Summary:Previous studies show that some ribosomal proteins perform immune effector functions via killing bacteria directly. However, it remains largely unknown about other effector functions of ribosomal proteins during a bacterial infection. In this study, we expressed and purified four ribosomal proteins of the amphioxus Branchiostoma japonicum, termed rBjRPS15, rBjRPS18, rBjRPS19 and rBjRPS30-precursor (rBjRPS30P). They all exhibited bactericidal activity against Gram-positive Staphylococcus aureus, and with the exception of rBjRPS19 and rBjRPS30P, were capable of killing Gram-negative Escherichia coli. Importantly, rBjRPS15, rBjRPS19 and rBjRPS30P were able to agglutinate S. aureus in the presence of Mg2+, but none of them could agglutinate E. coli even in the presence of Mg2+ or Ca2+. Moreover, the S. aureus agglutination was achieved by the binding of these three proteins to the peptidoglycan component of the bacterial cell wall. This is the first report showing that some ribosomal proteins possess bacterial agglutinating activity, and these data provide a new angle to the roles of ribosomal proteins in immune defense. •Amphioxus ribosomal protein RPS15, RPS18, RPS19 and RPS30P genes were cloned.•rBjRPS15, rBjRPS18, rBjRPS19 and rBjRPS30P showed strong bactericidal activity.•rBjRPS15, rBjRPS19 and rBjRPS30P exhibited bacterial agglutinating activity.•rBjRPS15, rBjRPS19 and rBjRPS30P agglutinate S. aureus via binding to bacterial PGN.
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2021.09.001