Factors influencing the response of spermatozoa to agitation stress: Implications for transport of extended boar semen

The shipping of liquid preserved semen is common practice in animal breeding and prior to cryopreservation for gene banking. Vibration emissions during transport may be harmful to spermatozoa. Therefore, strategies to minimize agitation-induced sperm injury are needed. The aim was to examine whether...

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Bibliographic Details
Published in:Theriogenology 2021-11, Vol.175, p.54-60
Main Authors: Paschoal, Aline FL, Luther, Anne-Marie, Jakop, Ulrike, Schulze, Martin, Bortolozzo, Fernando P., Waberski, Dagmar
Format: Article
Language:English
Subjects:
Boar spermatozoa
Semen agitation
Semen extender
Semen preservation
Storage temperature
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Summary:The shipping of liquid preserved semen is common practice in animal breeding and prior to cryopreservation for gene banking. Vibration emissions during transport may be harmful to spermatozoa. Therefore, strategies to minimize agitation-induced sperm injury are needed. The aim was to examine whether the type of semen extender, time after semen processing and the temperature in simulated transport conditions influence the response of boar spermatozoa to agitation stress. In Experiment 1, boar semen samples (n = 16) extended in Beltsville Thawing Solution (BTS) or Androstar Plus (APL) medium were filled in 90 mL tubes and shaken for 4 h at 200 rpm either at 22 °C or 17 °C. Samples were then stored at 17 °C for 144 h. In Experiment 2, semen samples (n = 11) extended in Androstar Premium were shaken either directly after filling at 22 °C or 20 h later after cooling to 5 °C. Samples were stored at 5 °C for 144 h. In Experiment 1, sperm motility and viability were lower (p 
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2021.08.033