In-vivo and in-silico studies of Eucalyptus kino polyphenolics: outstanding activity in quenching solid liver tumors through inhibition of MMP-9 and TGF-β gene expression

• Published in: Natural product research 2023-01, Vol.37 (2), p.343-347
• Main Authors: Sabry, Omar M, Sabry, Miral O, El-Sonbaty, Sawsan M, Meselhy, Khaled M
• Format: Article
• Language: English
• Subjects: Animals; Eucalyptus; Female; Gelatinase B; Gene Expression; Histopathology; Lead compounds; Liver; Liver Neoplasms; Low level; Matrix metalloproteinase; Matrix Metalloproteinase 9 - metabolism; Matrix metalloproteinases; Metalloproteinase; Mice; Molecular docking; Molecular Docking Simulation; Molecular modelling; Solid tumors; Transforming Growth Factor beta - metabolism; Transforming growth factor-b; Tumors; γ Radiation
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2021.1961254

As a result of our continuous research efforts to investigate the molecular mechanisms of Eucalyptus kino polyphenolics for healing liver solid tumors, in-vivo histopathological studies of the solid tumor tissues and in-silico molecular docking were carried out. Histopathology of female mice treated with a dose of 200 mg/kg Eucalyptus kino methanolic extract in combination with low level ionising γ-radiation (0.25 Gy) recovered a near-to-normal histological structure revealing a substantial number of cells with hyper-chromachia in nuclei, fibroblast lobules and necrotic cells. Analysis for matrix metalloproteinase-9 (MMP-9) by Western blot and also molecular docking study were conducted to evaluate and rank the potential of the individual components dominated in the extract to inhibit MMP-9 and TGF-β. The current study presents new lead compounds for the design of novel MMP-9 and TGF-β strong inhibitors of natural origin.