Fusarium Head Blight of Small Grains in Pennsylvania: Unravelling Species Diversity, Toxin Types, Growth, and Triazole Sensitivity

• Published in: Phytopathology 2022-04, Vol.112 (4), p.794-802
• Main Authors: Duffeck, Maíra R, Bandara, Ananda Y, Weerasooriya, Dilooshi K, Collins, Alyssa A, Jensen, Philip J, Kuldau, Gretchen A, Del Ponte, Emerson M, Esker, Paul D
• Format: Article
• Language: English
• Subjects: Fusarium - genetics; Genotype; Pennsylvania; Plant Diseases; Spores, Fungal; Triazoles - pharmacology; Triticum
• ISSN: 0031-949X; 1943-7684
• DOI: 10.1094/PHYTO-02-21-0070-R

is the main causal species of Fusarium head blight (FHB) globally. Recent changes in the trichothecene (toxin) types in the North American FHB pathogens support the need for continued surveillance. In this study, 461 isolates were obtained from symptomatic spikes of wheat, spelt, barley, and rye crops during 2018 and 2019. These were all identified to species and toxin types using molecular-based approaches. An additional set of 77 isolates obtained from overwintering crop residues during winter 2012 were molecularly identified to toxin types. A subset of 31 isolates (15 15-acetyl-deoxynivalenol [15ADON] and 16 3-acetyl-deoxynivalenol [3ADON]) were assessed for mycelial growth, macroconidia, perithecia, and ascospore production, and sensitivity to two triazoles. Ninety percent of isolates obtained from the symptomatic spikes ( = 418) belonged to , with four other species found at a lower frequency ( = 39). The isolates from symptomatic spikes were mainly of the 15ADON (95%), followed by 3ADON (4%), nivalenol (0.7%), and NX-2 (0.3%) toxin types. All isolates obtained from overwintering residue were of the 15ADON type. The toxin types could not be differentiated based on the multivariate analysis of growth and reproduction traits. All isolates were sensitive to tebuconazole and metconazole fungicides in vitro. This study confirms the dominance of and suggests ecological and environmental factors, to be further identified, that lead to similar composition of toxin types in the northern United States. Our results may be useful to assess the sustainability of FHB management practices and provide a baseline for future FHB surveys.