Increased transient receptor potential canonical 3 activity is involved in the pathogenesis of detrusor overactivity by dynamic interaction with Na+/Ca2+ exchanger 1

• Published in: Laboratory investigation 2022-01, Vol.102 (1), p.48-56
• Main Authors: Zhu, Jingzhen, Fan, Yi, Lu, Qudong, Yang, Yang, Li, Hui, Liu, Xin, Zhang, Hengshuai, Sun, Bishao, Liu, Qian, Zhao, Jiang, Yang, Zhenxing, Li, Longkun, Feng, Huan, Xu, Jie
• Format: Article
• Language: English
• Subjects: 101/1; 14/19; 14/34; 631/80/86/1999; 692/699/2768; 82/1; 9/74; Animal models; Bladder; Calcium (intracellular); Calcium ions; Cations; Excitability; Hyperactivity; Ion channels; Laboratory Medicine; Medicine; Medicine & Public Health; Muscles; Na+/Ca2+ exchanger; NCX1 protein; Pathogenesis; Pathology; Receptors; Rodents; Smooth muscle; Sodium; Synergistic effect; Therapeutic applications; Therapeutic targets; Transient receptor potential proteins
• ISSN: 0023-6837; 1530-0307
• DOI: 10.1038/s41374-021-00665-8

Transient receptor potential canonical 3 (TRPC3) is a nonselective cation channel, and its dysfunction is the basis of many clinical diseases. However, little is known about its possible role in the bladder. The purpose of this study was to explore the function and mechanism of TRPC3 in partial bladder outlet obstruction (PBOO)-induced detrusor overactivity (DO). We studied 31 adult female rats with DO induced by PBOO (the DO group) and 40 sham-operated rats (the control group). Here we report that the expression of TRPC3 in the bladder of DO rats increased significantly. Furthermore, PYR10, which can selectively inhibit the TRPC3 channel, significantly reduced bladder excitability in DO and control rats, but the decrease of the bladder excitability of DO rats was more obvious. PYR10 significantly reduced the intracellular calcium concentration in smooth muscle cells (SMCs) in DO and control rats. Finally, Na+/Ca2+ exchanger 1 (NCX1) colocalizes with TRPC3 and affects its expression and function. Collectively, these results indicate that TRPC3 plays an important role in the pathogenesis of DO through a synergistic effect with NCX1. TRPC3 and NCX1 may be new therapeutic targets for DO. The expression and functional levels of TRPC3, a nonselective cation channel, and NCX1, a Na+/Ca2+ exchanger, are increased in the bladders of rats with partial bladder outlet obstruction-induced detrusor overactivity. The synergistic effects of TRPC3 and NCX1 significantly increase the concentration of Ca2+i in smooth muscle cells, which induces bladder hyperactivity in this animal model of overactive bladder. TRPC3 and NCX1 may be new therapeutic targets for detrusor overactivity.