CKIP-1 contributes to osteogenic differentiation of mouse bone marrow mesenchymal stem cells

Osteogenesis greatly depends on the differentiation of bone marrow mesenchymal stem cells (BMSCs). CKIP-1 is considered to be a negative regulator of BMSCs. We established a  knockout mouse model, then isolated and cultured BMSCs from wild-type and knockout groups. Our data demonstrated that knockou...

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Bibliographic Details
Published in:Regenerative medicine 2021-09, Vol.16 (9), p.847-859
Main Authors: Niu, Qiannan, Shen, Shuning, He, Jiaojiao, Wang, Lei
Format: Article
Language:English
Subjects:
Animals
Bone and Bones
Bone Marrow Cells
bone marrow mesenchymal stem cells
Carrier Proteins
Cell Differentiation
Cells, Cultured
CKIP-1
MAPK signal pathway
Mesenchymal Stem Cells
Mice
Osteogenesis
osteogenic differentiation
osteoporosis
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Summary:Osteogenesis greatly depends on the differentiation of bone marrow mesenchymal stem cells (BMSCs). CKIP-1 is considered to be a negative regulator of BMSCs. We established a  knockout mouse model, then isolated and cultured BMSCs from wild-type and knockout groups. Our data demonstrated that knockout significantly increased bone structure in the experimental mouse model and enhanced BMSC proliferation. knockout contributed to osteoblastic and adipogenic differentiation. Furthermore, CKIP-1 regulated osteogenesis in BMSCs via the MAPK signaling pathway, and BMSCs from the  knockout mice were effective in repairing the skull defect null mice. Our results concluded that silencing of promoted osteogenesis in experimental mice and increased BMSCs differentiation via upregulation of the MAPK signaling pathway.
ISSN:1746-0751
1746-076X
DOI:10.2217/rme-2020-0119