Exogenous addition of silicon alleviates metsulfuron methyl induced stress in wheat seedlings

• Published in: Plant physiology and biochemistry 2021-10, Vol.167, p.705-712
• Main Authors: Jain, Shruti, Rai, Padmaja, Singh, Jaspreet, Singh, Vijay Pratap, Prasad, Rajendra, Rana, Shweta, Deshmukh, Rupesh, Tripathi, Durgesh Kumar, Sharma, Shivesh
• Format: Article
• Language: English
• Subjects: Antioxidant enzymes; Herbicide toxic effect; Metsulfuron methyl; Reactive oxygen species (ROS); Silicon; Triticum aestivum
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2021.07.031

Uncontrolled application of herbicides in the agricultural field poses a severe risk to crops by affecting their yields. Therefore, methods are required to reduce the toxic effects of herbicides in plants. Studies indicate that silicon (Si) provides tolerance and enhances defence mechanism of the plant against abiotic stress. But its role in alleviating Metsulfuron methyl (Meth) herbicide induced toxicity in wheat seedlings is still not known. This study highlighted the potential of exogenous addition of Si in the alleviation of toxic effect of Meth herbicide in wheat seedlings. The exposure of wheat seedlings to Meth herbicide reduced the growth, photosynthetic pigments, antioxidant enzyme activity and nitric oxide (NO) content. Further, Meth herbicide also increased cell death and decreased cell viability in root tips. However, addition of Si reversed Meth-induced these alterations. Moreover, Si also activates antioxidant system which helps in scavenging of free radicals generated under Meth herbicide stress in wheat seedlings. Application of Si to Meth treated wheat seedlings also up-regulated silicon transporter gene Lsi1 (silicon influx transporter) and some of the antioxidant enzyme genes. All together, the data indicate that Si has capability of alleviating Meth herbicide stress in wheat seedlings but it appears that endogenous NO has a positive role in this endeavour of Si. •Metsulfuron methyl herbicide significantly reduced growth of wheat plants.•But, addition of silicon alleviated metsulfuron methyl induced stress in wheat.•Furthermore, supplementation of Si up -regulated the expression level of both stress associated genes and Si transporters gene (Lsi1) under metsulfuron methyl stress.•NO is involved in Si governed alleviation of metsulfuron methyl induced stress in wheat.