Design and Production of Customizable and Highly Aligned Fibrillar Collagen Scaffolds

• Published in: ACS biomaterials science & engineering 2023-07, Vol.9 (7), p.3962-3971
• Main Authors: Martin, Cassandra L., Zhai, Chenxi, Paten, Jeffrey A., Yeo, Jingjie, Deravi, Leila F.
• Format: Article
• Language: English
• Subjects: Collagen; Collagen Type I; Fibrillar Collagens; Tissue Engineering
• ISSN: 2373-9878; 2373-9878
• DOI: 10.1021/acsbiomaterials.1c00566

The ability to fabricate anisotropic collagenous materials rapidly and reproducibly has remained elusive despite decades of research. Balancing the natural propensity of monomeric collagen (COL) to spontaneously polymerize in vitro with the mild processing conditions needed to maintain its native substructure upon polymerization introduces challenges that are not easily amenable with off-the-shelf instrumentation. To overcome these challenges, we have designed a platform that simultaneously aligns type I COL fibrils under mild shear flow and builds up the material through layer-by-layer assembly. We explored the mechanisms propagating fibril alignment, targeting experimental variables such as shear rate, viscosity, and time. Coarse-grained molecular dynamics simulations were also employed to help understand how initial reaction conditions including chain length, indicative of initial polymerization, and chain density, indicative of concentration, in the reaction environment impact fibril growth and alignment. When taken together, the mechanistic insights gleaned from these studies inspired the design, iteration, fabrication, and then customization of the fibrous collagenous materials, illustrating a platform material that can be readily adapted to future tissue engineering applications.