Nano‐liquid chromatography with a new nano‐structured monolithic nanocolumn for proteomics analysis

Herein, we report the preparation and application of a new nano‐structured monolithic nanocolumn based on modified graphene oxide using narrow fused silica capillary column (e.g., 50 μm internal diameter). The nanocolumn was prepared by an in situ polymerization using butyl methacrylate, ethylene di...

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Bibliographic Details
Published in:Journal of separation science 2021-11, Vol.44 (21), p.3996-4004
Main Authors: Günyel, Zeynep, Aslan, Hakiye, Demir, Nurullah, Aydoğan, Cemil
Format: Article
Language:English
Subjects:
Alkylbenzenes
Chromatography, Liquid - methods
Cytochromes
Dimethylformamide
Fused silica
Graphene
graphene oxide
Graphite - chemistry
Hydrophobicity
Liquid chromatography
Macromolecules
monoliths
Nanoparticles
Nanotechnology - methods
nano‐liquid chromatography
Peptides
Plates
Polymerization
Propylbenzene
protein separation
Proteins
Proteins - analysis
Proteins - chemistry
Proteins - isolation & purification
Proteomics
Proteomics - methods
Resolution
Silicon dioxide
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Summary:Herein, we report the preparation and application of a new nano‐structured monolithic nanocolumn based on modified graphene oxide using narrow fused silica capillary column (e.g., 50 μm internal diameter). The nanocolumn was prepared by an in situ polymerization using butyl methacrylate, ethylene dimethacrylate, and methacryloyl graphene oxide nanoparticles. Dimethyl formamide and water were used as the porogenic solvent. After polymerization, the obtained nanocolumn was coated with dimethyloctadecylchlorosilane in order to enhance the hydrophobicity. Both isocratic and gradient nano‐liquid chromatographic separations for small molecules (e.g., alkylbenzenes) and macromolecules (e.g., intact proteins) were performed. Theoretical plates number up to 3600 plates/m in isocratic mode for propylbenzene were achieved. It was demonstrated that the feasibility of graphene oxide modified monolithic nanocolumn for high‐efficiency and high‐throughput nanoscale proteomics analysis. The high resolving power of monolithic nanocolumn yielded sensitive protein separation with narrower peak width while a high‐resolution analysis of peptides from trypsin‐digested cytochrome C could be obtained. Graphene oxide based monolithic nanocolumns are promising and can allow to powerful tools for trace proteom sample analysis.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.202100454