Bacteriophage mediated control of necrotic enteritis caused by C. perfringens in broiler chickens

• Published in: Veterinary research communications 2021-12, Vol.45 (4), p.409-421
• Main Authors: Hosny, Reham A., Gaber, Ahmed F., Sorour, Hend K.
• Format: Article
• Language: English
• Subjects: Animals; Bacteriophages - physiology; Biomedical and Life Sciences; Cecum; Chickens; Clostridium Infections - microbiology; Clostridium Infections - veterinary; Clostridium perfringens - physiology; Clostridium perfringens - virology; Contractility; Dogs; Enteritis; Enteritis - microbiology; Life Sciences; Mortality; Necrosis - microbiology; Original Article; pH effects; Phages; Poultry; Poultry Diseases - microbiology; Thermal stability; Transmission electron microscopy; Veterinary Medicine/Veterinary Science; Zoology
• ISSN: 0165-7380; 1573-7446
• DOI: 10.1007/s11259-021-09821-3

In Egypt, little attention has been paid to the isolation and application of C. perfringens phages for treating necrotic enteritis at the farm level. This study aims to evaluate the efficiency of the podovirus C. perfringens phage in treating necrotic enteritis in broiler chickens. Accordingly, C. perfringens phage was isolated from cecal samples of apparently healthy chickens and characterized by transmission electron microscopy, thermal stability test, and pH stability test. Commercial 14-day-old Arbor Acres broiler chickens were allocated to three groups: group Ӏ received BHI broth and assigned as a negative control, group П served as a positive control group that was challenged with C. perfringens via oral gavage for four successive days, and group Ш was administrated six phage doses on several occasions after oral gavage challenge with C. perfringens . Daily clinical symptoms and mortality were recorded. At three-time intervals, necrotic enteritis lesions were scored. Cecal samples were examined for re-isolation and counting of C. perfringens. The isolated C. perfringens phage was a podovirus with an icosahedral head diameter of 78.7 nm and a short non-contractile tail length of 22.2 nm. It remained stable for 60 min at 30 °C and 50 °C at pH values of 2, 4, 8, and 10. The phage-treated group (Ш) showed mild gross lesions with a lower mortality rate and reduced colony-forming units than the positive control group (П). The findings revealed that the isolated C. perfringens phage effectively treated experimental necrotic enteritis in broiler chickens.