UVPD spectroscopy of differential mobility-selected prototropic isomers of protonated adenine

Two prototropic isomers of adenine are formed in an electrospray ion source and are resolved spatially in a differential mobility spectrometer before detection in a triple quadrupole mass spectrometer. Each isomer is gated in CV space before being trapped in the linear ion trap of the modified mass...

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Bibliographic Details
Published in:Physical chemistry chemical physics : PCCP 2021-09, Vol.23 (35), p.19892-199
Main Authors: Heldmaier, Fiorella Villanueva, Coughlan, Neville J. A, Haack, Alexander, Huard, Rebecca, Guna, Mircea, Schneider, Bradley B, Le Blanc, J. C. Yves, Campbell, J. Larry, Nooijen, Marcel, Hopkins, W. Scott
Format: Article
Language:English
Subjects:
Adenine
Ion sources
Ionograms
Ions
Isomers
Optical Parametric Oscillators
Parametric amplifiers
Photodissociation
Quadrupoles
Spectra
Spectrum analysis
Tautomers
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Summary:Two prototropic isomers of adenine are formed in an electrospray ion source and are resolved spatially in a differential mobility spectrometer before detection in a triple quadrupole mass spectrometer. Each isomer is gated in CV space before being trapped in the linear ion trap of the modified mass spectrometer, where they are irradiated by the tuneable output of an optical parametric oscillator and undergo photodissociation to form charged fragments with m / z 119, 109, and 94. The photon-normalised intensity of each fragmentation channel is measured and the action spectra for each DMS-gated tautomer are obtained. Our analysis of the action spectra, aided by calculated vibronic spectra and thermochemical data, allow us to assign the two signals in our measured ionograms to specific tautomers of protonated adenine. Differential mobility spectrometry is used to separate prototropic isomers of protonated adenine prior to laser spectroscopic investigation.
ISSN:1463-9076
1463-9084
DOI:10.1039/d1cp02688g